Comparative classification of Acinetobacter baumannii strains using seven different typing methods

https://doi.org/10.1016/S0934-8840(11)80707-3Get rights and content

Summary

A group of 49 Acinetobacter baumannii strains obtained from several hospital outbreaks and some sporadic cases were typed by biotyping, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), plasmid typing, multilocus enzyme electrophoresis, whole-cell protein profile, and Fourier-transform infrared (FT-IR) spectroscopy. All these methods have shown a high degree of reproducibility and are capable of recognising strains from the same epidemiological event. However, their power to discriminate between epidemiologically unrelated strains varies, with PFGE being superior to the other methods investigated. FT-IR spectroscopy, which has not yet been used for typing of Acinetobacter strains, proved to be a very rapid and highly reproducible method, but was somewhat limited in its discriminating power.

References (26)

  • P.J. M. Bouvet et al.

    Species, biotype, and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains

    J. Clin. Microbiol

    (1990)
  • L. Dijksboorn et al.

    Correlation of typing methods for Acinetobacter isolated from hospital outbreaks

    J. Clin. Microbiol

    (1993)
  • P. Gerner-Smidt

    Ribotyping of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex

    J. Clin. Microbiol

    (1992)
  • Cited by (23)

    • Phylogenetic analysis of carbapenem-resistant Acinetobacter baumannii isolated from different sources using Multilocus Sequence Typing Scheme

      2021, Infection, Genetics and Evolution
      Citation Excerpt :

      A lot of Acinetobacter strain typing methods with different intrinsic degrees of resolution have been used (Zarrilli et al., 2013). These includes traditional typing methods such as serotyping multilocus enzyme electrophoresis, and DNA-based methods, including repetitive extragenic palindromic sequence-based PCR, amplified ribosomal DNA restriction analysis (ARDRA), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and ribotyping (Traub, 1989; Seltmann et al., 1995; Huys et al., 2005; Vaneechoutte et al., 1995; Misbah et al., 2005). However, these methods lack universal standard and portability, and therefore it is difficult to compare results obtained in different laboratories and are also labour intensive and time consuming (Ecker et al., 2006).

    • Applying Fourier-transform infrared spectroscopy and chemometrics to the characterization and identification of lactic acid bacteria

      2011, Vibrational Spectroscopy
      Citation Excerpt :

      For taxonomy studies the IR spectrum of intact microbial cells constitutes an image of their overall chemical composition, but clearly some compounds and structures (mainly cell membrane and wall) have a decisive influence on the spectrum. We illustrate in our study that a relationship between cell components and FT-IR exist, as it is also shown to a different degree in other studies [30,32,42–45]. Sometimes the taxonomic significance of FT-IR data appears to be limited although it supplements very significant information [4,46].

    • Rapid identification of Candida species by FT-IR microspectroscopy

      2005, Biochimica et Biophysica Acta - General Subjects
    View all citing articles on Scopus
    a

    Dr. G. Seltmann, Robert-Koch-Institut, Bereich Wernigerode, Burgstr. 37, D-38855 Wernigerode

    View full text