IL-4 Decreases the Expression of the Monocyte Differentiation Marker CD14, Paralleled by an Increasing Accessory Potency

doi:10.1016/S0171-2985(11)80209-3

Immunobiology

Volume 182, Issue 5, August 1991, Pages 449-464

https://doi.org/10.1016/S0171-2985(11)80209-3 Get rights and content

Abstract

IL-4 has been found to affect the phenotype and a variety of functions of human monocytes and macrophages and has been discussed as a monocyte activating protein along with other cytokines, such as IL-1 and IL-6. In this study we compared the effects of the cytokines IL-1, IL-6, IL-4, and a combination of IL-1 and IL-6 on the expression of the CD14 antigen, the FcIIIg receptor molecule CD16 and the MHC-class II molecules HLA-DR and HLA-DP. These molecules represent characteristic monocyte surface markers. Furthermore, the CD14 molecule has been described as a surface antigen of high in vivo relevance representing an indirect receptor for LPS. We further analyzed the effect of IL-4 on monocytes and macrophages with respect to their accessory function to initiate T-lymphocyte proliferation. Human peripheral blood monocytes strongly express the antigen CD14 and maintain it as a stable surface molecule during their differentiation to macrophages. Flow cytometry analysis of cultured monocytes demonstrated that cells incubated in the presence of IL-4, but not IL-1 and/or IL-6 revealed a reduced expression of the CD14 antigen in a dose- and time-dependent manner. After 3 days IL-4 treated cells were virtually CD14-negative. At the same time the expression of the CD 16 antigen (FcRIIIg) was also strongly reduced, whereas the treatment with IL-4 led to an increased expression of MHC class II antigens such as HLA-DR and HLA-DP. The spontaneous low expression of HLA-DQ antigen on monocytes was not affected by any of the cytokines. Functionally, IL-4 treated CD14-negative monocytes exhibited a more than 2-fold higher activity to stimulate an accessory cell-dependent T cell proliferation. This was found in a mitogenic assay and in MLC when compared to monocytes cultured in the absence of IL-4. These observations provide further evidence that IL-4 is a major modulator of monocyte surface antigen expression. Moreover, IL-4 has an enhancereffect on monocytes as accessory cells and therefore may be of considerable importance as a regulatory factor during monocyte development to accessory cells. Inasmuch as the CD14 molecule functions as a receptor for LPS-binding protein, our results suggest that IL-4 might also play an important regulatory role in processes initiated by bacterial lipopolysaccharides during inflammation and sepsis.

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²: Dr. J.H Peters, Abteilung Immunologie, Kreuzbergring 57, 3400 Güttingen, Germany

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IL-4 Decreases the Expression of the Monocyte Differentiation Marker CD14, Paralleled by an Increasing Accessory Potency

Abstract

Immunol. Today

Immunol. Today

Hum. Immunol.

Immunol. Today

Molec. Immunol.

Molec. Immunol.

Identification of a T cell derived B cell growth factor distinct from interleukin-2

J. Exp. Med.

Increased expression of la antigens on resting B cells: an additional role for B-cell growth factor

Proc. Natl. Acad. Sci. USA

Interleukin-induced increase in Ia expression by normal mouse B cells

J. Exp. Med.

Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T cell and mast-cell stimulating activities

Proc. Natl. Acad. Sci. USA

Modulation of phenotypic and functional properties of human peripheral blood monocytes by IL-4

J. Immunol.

B cell stimulatory factor-1 (interleukin 4) activates macrophages for increased tumoricidal activity and expression of la antigens

J. Immunol.

Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-α

J. Immunol.

Effects of recombinant B cell growth factor 1 on a macrophage cell line

J. Leukocyte Biol.

IL-4 and granulocyte macrophage colony-stimulating factor selectively increase HLA-DR and HLA-DP antigens but not HLA-DQ antigens on human monocytes

J. Immunol.

Magnitude of response of histocompatibility-restricted T-cell clones is a function of the product of the concentrations of antigen and la molecules

Proc. Natl. Acad. Sci. USA

Synergistic activation of human T cells by interleukin 1 and interleukin 6

Eur. J. Immunol.

Interleukin-6 (IL-6) and interleukin-1 (IL-1) enhance the accessory activity of human blood monocytes during differentiation to mature macrophages

J. Immunol.

Antigens on human monocytes identified by monoclonal antibodies

J. Immunol.

Accessory cell requirements for the mixed leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells

Cell. Immunol.

Relative efficacy of human monocytes and dendritic cells as accessory cells for T-cell replication

J. Exp. Med.

CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein

Science

Differentiation of human monocytes into accessory cells at serum-free conditions

Europ. J. Cell Biol.