Expression of novel alternatively spliced isoforms of the oct-1 transcription factor

doi:10.1016/S0167-4781(98)00280-2

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

Volume 1444, Issue 2, 16 February 1999, Pages 295-298

https://doi.org/10.1016/S0167-4781(98)00280-2 Get rights and content

Abstract

Analysis of the alternatively spliced isoforms of the human and mouse oct-1 genes, combined with their exon–intron structure, show a high level of evolutionary conservation between these two species. The differential expression of several oct-1 isoforms was examined by reverse transcription–polymerase chain reaction performed on the 3′ region of the murine oct-1 cDNA. Variations in the relative levels and patterns of expression of the isoforms were found among different tissues. Three novel isoforms originating from the 3′-distal region of oct-1, were isolated and sequenced: Two were derived from testis, and one from myeloma cells. Splicing out of different exons as revealed in the structure of these isoforms results in reading frameshifts that presumably lead to the expression of shortened Oct-1 proteins, with distinct C-terminal tails. Altogether, six out of the eight known murine oct-1 isoforms may have distinct C-termini, implying that these multiple tails have different functional roles in cellular differentiation and physiology.

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¹: The sequence data reported in this paper have been submitted to the EMBL/GenBank under accession numbers AFO95458, AFO95459, AFO95460.

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Short sequence-paperExpression of novel alternatively spliced isoforms of the oct-1 transcription factor1

Abstract

Curr. Opin. Cell Biol.

Biochim. Biophys. Acta

Cell

Biochim. Biophys. Acta

Genomics

Short sequence-paper
Expression of novel alternatively spliced isoforms of the oct-1 transcription factor¹