Elsevier

Journal of Chromatography A

Volume 557, 20 September 1991, Pages 507-513
Journal of Chromatography A

Short Cummunication
Cyclooxygenase and lipoxygenase arachidonic acid metabolism by monocytes from human immune deficiency virus-infected drug users

https://doi.org/10.1016/S0021-9673(01)87159-4Get rights and content

Abstract

Prostaglandin E2, thromboxane B2 and leokotriene B4 monocyte production have been determined in human immune deficiency virus (HIV)-infected drug users (n = 36) and healthy subjects (n = 29). Eiscosanoids were extracted from the incubates using C18 solid-phase cartridges and determined by radioimmunoassay. An enhanced production of prostaglandin E2 and thromboxane B2 was detected in monocytes from HIV-positive drug users whether or not they had bees previously stimulated with zymosan. Concomitant leukotriene B4 increases were not observed. The result reported in this paper indicate that altered cyclooxygenase arachidonic acid metabolism in monocytes from HIV-infected drug users is associated with the severe cellular immunodysfunction characteristic of AIDS. In contracts, leukotriene B4 does not seem to play a role in AIDS-associated immunosuppression.

References (15)

  • J.S. Goodwin et al.

    J. Clin. Immunol. Immunopathol.

    (1980)
  • E. Gelpí et al.

    J. Chromatogr.

    (1989)
  • J. Abian et al.

    J. Chromatogr.

    (1991)
  • J.I. Kurland et al.

    J. Exp. Med.

    (1987)
  • S. Gartner et al.

    Science

    (1986)
  • E. Fernandez-Cruz et al.

    AIDS

    (1989)
  • M. Rola-Pleszczynski et al.

    Biochem. Biophys. Res. Commun.

    (1982)
There are more references available in the full text version of this article.

Cited by (26)

  • PGJ<inf>2</inf> antagonizes NF-κB-induced HIV-1 LTR activation in colonic epithelial cells

    2008, Virology
    Citation Excerpt :

    During the time course of inflammation, the prostaglandins profile shifts from the predominantly pro-inflammatory PGE2 to the anti-inflammatory PGJ2, which is the end product metabolite of PGD2 (Gilroy et al., 1999; Ianaro et al., 2001; Kapoor et al., 2005a, 2005b). Pro-inflammatory molecules such as PGE2 are up-regulated during HIV-1 infection (Griffin et al., 1994; Ramis et al., 1991) leading to an imbalance in PGJ2 production. Given that the cyclopentone prostaglandin PGJ2 has potent anti-inflammatory properties, we examined whether the addition of PGJ2 could inhibit HIV-1 transcription in intestinal epithelial cells.

  • Intracerebral HIV-1 glycoprotein 120 produces sickness behavior and pituitary-adrenal activation in rats: Role of prostaglandins

    2002, Brain, Behavior, and Immunity
    Citation Excerpt :

    The ex vivo technique reduces the contribution of the decapitation, because in this method the conversion of endogenous arachidonate to prostaglandins in brain tissues is measured after washing the tissues (Weidenfeld, Lysy, & Shohami, 1987). Although the finding that individuals infected with HIV have significantly higher levels of PGE2 than uninfected individuals is well established (Foley, Kazazi, Biti, Sorrell, & Cunningham, 1992; Griffin, Wesselingth, & McArthur, 1994; Ramis et al., 1991; Tarter et al., 1997), the role of gp120 in this elevation has been debated. In several studies, incubation of monocytes (Wahl et al., 1989), bronchial alveolar macrophages (Denis, 1994), or cultured astroglial cells (Mollace et al., 1994) with gp120 was found to induce the production of prostaglandins.

  • Prostaglandin E<inf>2</inf> inhibits replication of HIV-1 in macrophages through activation of protein kinase A

    2002, Cellular Immunology
    Citation Excerpt :

    Experimental infection of PBM with HIV-1 results in an increase in the production of PGE2 relative to uninfected cultures [15]. In vitro production of thromboxane (TX) B2 by PBM of AIDS patients is also increased relative to normal controls [16,17]. The HIV-1 envelope glycoprotein gp120 also induces the production of PGE2 by normal PBM [15].

View all citing articles on Scopus
View full text