Thyroid hormone regulation by stress and behavioral differences in adult male rats
Research highlights
► In male rats, acute stress causes a decrease in thyroid hormones within 120 min. ► Hormone changes were not associated with alterations in PVN TRH mRNA levels. ► Thyroid hormone levels are correlated with an animal's exploratory drive.
Section snippets
Animals
Seventy-five adult male Sprague–Dawley rats (Charles River Laboratories), body weight 250–310 g at the beginning of the stress sessions were used for this study. All animals were housed two per tub in 40 × 18 × 20 cm tubs. Lights were on 0730 to 1930 h and food and water were available ad libitum. All animals were acclimated to the housing facility for at least 5 days before testing. Within each tub, each animal was randomly assigned to either the inescapable tail-shock stress group or control group.
Stress paradigm
A
Experiment 1
As shown in Fig. 1, we found that 80 trials of inescapable tail-shock stress caused a decrease in peripheral TH levels. Specifically for T3, a two-way ANOVA revealed a significant main effect for stress (F(1, 66) = 8.75, p < .01). Bonferroni-corrected post-hoc tests indicated that this decrease was significant at 120 min post-stress initiation (Fig. 1A). Stress also caused a significant decrease in circulating levels of T4, indicated by a significant stress x time interaction (F(4, 66) = 3.32, p < .01;
Discussion
In the current series of experiments, we sought to further characterize acute stress-induced changes in HPT axis function and to also determine if TH levels are correlated with an animals' spontaneous behavior. In Experiment 1 we replicated our previous results, showing that acute stress can cause a decrease in circulating T3 levels within 120 min post-stress initiation (Helmreich et al., 2006). We extended these findings by demonstrating that acute stress also causes a decrease in circulating T4
Acknowledgments
We would like to thank the vivarium staff at the University of Rochester for their excellent animal care and James Walton for his excellent technical assistance. Daniel Tylee earned course credit and received an undergraduate research grant from SUNY Geneseo to support his work on these experiments. This work was supported by the Department of Psychiatry at the University of Rochester, and grant MH1R03MH080789 to DLH. The authors appreciate the thoughtful comments from Drs. R. Ader, J. Fudge,
References (61)
- et al.
Mechanisms of nongenomic actions of thyroid hormone
Front. Neuroendocrinol.
(2008) - et al.
Negative feedback regulation of hypophysiotropic thyrotropin-releasing hormone (TRH) synthesizing neurons: role of neuronal afferents and type 2 deiodinase
Front. Neuroendocrinol.
(2007) - et al.
Depression, anxiety, health-related quality of life, and disability in patients with overt and subclinical thyroid dysfunction
Arch Med Res
(2006) Drugs that suppress TSH or cause central hypothyroidism
Best Pract Res Clin Endocrinol Metab
(2009)- et al.
Peripheral triiodothyronine (T3) levels during escapable and inescapable shock
Physiol. Behav.
(2006) - et al.
Neurocircuitry of stress: central control of the hypothalamic–adrenocortical axis
Trends Neuroscience
(1997) - et al.
Polymorphisms in the serotonin reuptake transporter gene modify the consequences of social status on metabolic health in female rhesus monkeys
Physiol. Behav.
(2008) - et al.
The TRH neuron: a hypothalamic integrator of energy metabolism
Prog Brain Res
(2006) - et al.
The role and potential sites of action of thyroid hormone in timing the onset of puberty in male primates
Brain Res
(2010) - et al.
The concept of allostatis in biology and biomedicine
Horm. Behav.
(2003)
Regulation of the hypothalamic thyrotropin releasing hormone (TRH) neuron by neuronal and peripheral inputs
Front. Neuroendocrinol.
The regulatory role of neurotensin on the hypothalamic–anterior pituitary axons: emphasis on the control of thyroid-related functions
Neuropeptides
Administration of thyroid hormone increases reelin and brain-derived neurotrophic factor expression in rat hippocampus in vivo
Brain Res
Amphibian metamorphosis as a model for the developmental actions of thyroid hormone
Mol. Cell. Endocrinol.
Narrow individual variations in serum T(4) and T(3) in normal subjects: a clue to the understanding of subclinical thyroid disease
J. Clin. Endocrinol. Metab.
Effect of acute and chronic psychogenic stress on corticoadrenal and pituitary–thyroid hormones in male rats
Hormone Res
Werner's and Ingbar's the thyroid: a fundamental and clinical text: Lippincott Williams and Wilkins
Analysis of pulsatile secretion of thyrotropin and growth hormone in the hypothyroid rat
Endocrinology
Thyroid hormone action: nongenomic modulation of neuronal excitability in the hippocampus
J. Neuroendocrinol.
Age and gender influence basal and stress-modulated hypothalamic–pituitary–thyroidal function in Fisher 344/N rats
Neuroendocrinology
Ancient origins of human developmental plasticity
Am. J. Hum. Biol.
Neuropsychiatric aspects of hypothyroidism and treatment reversibility
Minerva Endocrinol
The regulation of seasonal changes in food intake and body weight
J. Neuroendocrinol.
Conservation-withdrawal: a primary regulatory process for organismic homeostatsis
CIBA Found. Symp.
Ascending brainstem pathways are not involved in lipopolysaccharide-induced suppression of thyrotropin-releasing hormone gene expression in the hypothalamic paraventricular nucleus
Endocrinology
Cellular and molecular basis of deiodinase-regulated thyroid hormone signaling
Endocr. Rev.
Pulsatile thyrotropin secretion in patients with Addison's disease during variable glucocorticoid therapy
J. Clin. Endocrinol. Metab.
The effect of stressor controllability on stress-induced neuropeptide mRNA expression within the paraventricular nucleus of the hypothalamus
J. Neuroendocrinol.
Relation between the hypothalamic–pituitary–thyroid (HPT) axis and the hypothalamic–pituitary–adrenal (HPA) axis during repeated stress
Neuroendocrinology
The thyrotropin-releasing hormone-degrading ectoenzyme: the third element of the thyrotropin-releasing hormone-signaling system
Thyroid
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