Fig. 1. Phylogeny of partial nucleotide sequence of RABV N gene isolated during the Augusto Correa outbreak occurred in 2005. Bayesian method and bootstrap analysis were used for three constructions. Bootstrap percentage values for ML method and Bayesian values (inside parenthesis) are placed over each main group node. Arrows are indicating the exact position of bootstrap values. Number under the scale bar corresponds to 3% nucleotide sequence divergence.

Fig. 2. Schematic rabies virus tree rooted with AVO1 and CVS strains indicating the starlike evolution. Divergence times (upper and lower values) are shown over all nodes of the tree.

Fig. 3. Geographic position of the places where the RABV strains used for phylogenetic analysis was isolated. Black dot corresponds to Augusto Correa Municipality, Pará state [PA]. Triangles represent other municipalities of different Brazilian states which are highlighted in light gray (TO: Tocantins; MT: Mato Grosso; GO: Goias; MG: Minas Gerais; SP: São Paulo). Areas highlighted in dark gray represent the Marajo region in which Portel municipality is included. Dashed areas correspond to the northeast region of Pará state including Augusto Correa and Viseu municipalities. ?: unknown municipalities.

Analysis of maximum-likelihood ratio for the molecular clock hypothesis

ML = maximum-likelihood method; MLK = maximum-likelihood method enforcing a molecular clock.

Rabies virus strains used for genetic characterization according with its host association, geographic origin, and year of isolation