Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Oral medicineIdentification of oral bacteria in blood cultures by conventional versus molecular methods
Section snippets
Patient recruitment and sampling
We enrolled 30 essentially healthy adult subjects who required extraction of a single tooth and who had at least 10 remaining teeth. These subjects were part of a larger study concerning bacteremia resulting from dental procedures. Exclusion criteria included taking systemic antibiotics within the previous 2 weeks, active bacterial infectious disease, immunocompromise (e.g., organ transplant, HIV), facial cellulites, and any manipulation of the gingiva within 1 h before the study (e.g., eating,
Results
All but 1 baseline blood samples were negative for bacterial growth. Positive cultures from postextraction blood samples were selected from 22 subjects for further subculturing. These cultures were from blood samples drawn at different time points. Fifty-eight bacterial isolates were analyzed by both rapid biochemical tests and 16S rRNA gene sequencing. Of these, 10 (17%) isolates were shown to render concordant identification, 32 (55%) belonged to the same genus but different species, and 16
Discussion
Identification of bacterial isolates from blood samples is still largely based on morphologic characteristics, growth conditions, and physiologic and biochemical properties.4 The Remel RapID identification system is a qualitative micromethod providing an easy way to identify bacteria from various families based on phenotypic characteristics. Similar to other computerized identification systems, Remel RapID comprises a battery of biochemical tests. The +/− reaction of the tests generates a
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Cited by (0)
Supported by NIDCR/NIH grant no. R01 DE13559-01 and a grant from the Health Services Foundation, Carolinas Medical Center, Charlotte, NC.