Oral and maxillofacial pathology
Expression of extracellular matrix metalloproteinase inducer in odontogenic cysts

https://doi.org/10.1016/j.tripleo.2008.01.021Get rights and content

Objective

Extracellular matrix metalloproteinase inducer (EMMPRIN) is known to induce matrix metalloproteinase (MMP) production. The expression of EMMPRIN in odontogenic cysts has not been previously studied. This study was done to determine the presence and the variability of EMMPRIN expression in various types of odontogenic cysts.

Study design

An immunohistochemical study using a polyclonal anti-EMMPRIN antibody was done using 48 odontogenic cyst cases: 13 odontogenic keratocysts (OKCs), 18 dentigerous cysts (DCs), and 17 periapical cysts (PAs). Twelve cases of normal dental follicles (DFs) were also included in this study for comparison.

Results

EMMPRIN immunoreactivity was detected in all of the cysts and DFs studied. In odontogenic cysts, EMMPRIN immunoreactivity was generally higher in basal cells than in suprabasal cells. The overall EMMPRIN expression in the epithelial lining of the 3 different types of odontogenic cyst was significantly higher than in the DFs. Overall EMMPRIN expression was also found to be significantly higher in the epithelial lining of OKCs than in the other types of cysts.

Conclusion

This study confirmed that EMMPRIN is present in odontogenic cysts and DFs. The higher EMMPRIN expression in OKCs suggests that it may be involved in the aggressive behavior of this type of cyst.

Section snippets

Tissue specimens

The original hematoxylin and eosin (H&E)–stained sections and formalin-fixed paraffin-embedded specimen blocks of all OKCs, DCs, and PAs diagnosed between January 2004 and October 2005 were retrieved from the Department of Histopathology, Amiri Hospital, Kuwait City, Kuwait. The H&E sections were reviewed to confirm the diagnosis. Cysts not satisfying the histologic criteria outlined by the World Health Organization classification,18 intensely inflamed OKCs and DCs, orthokeratinized OKCs, OKCs

Results

Of the 48 cysts assessed, 29 (60.4%) were found in males and 19 (39.6%) were found in females. Thirteen cysts (27.1%) were seen in the maxilla and 35 (72.9%) in the mandible. The median patient age was 27 years (range, 6-62 years; Table I).

EMMPRIN was detected in all of the odontogenic cysts and DFs. EMMPRIN staining was detected in the cell membrane of both the basal and the suprabasal odontogenic epithelial cells (Fig. 1). EMMPRIN showed continuous and uniform expression in both the basal and

Discussion

The MMPs are a family of enzymes that have the ability to degrade different components of the extracellular matrix.1 More than 20 different MMPs have been identified.2 The MMPs are classified based on structure and substrate specificity into subgroups of collagenases, gelatinases, stromelysins, matrilysins, and membrane-type MMPs (MT-MMPs).2 The MMPs play an important role in several physiologic and pathologic processes, including embryonic development, morphogenesis, reproduction, and tissue

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