Other tissue/organ transplantationCould the coculture of skeletal myoblasts and mesenchymal stem cells be a solution for postinfarction myocardial scar?
Section snippets
Methods
The isolation of SM from sacrificed neonatal rats used the enzymatic dissociation technique reported by Delaporte et al,9 employing muscular fragments from the four limbs. The isolation of MSC of BM was performed by two different harvesting techniques; a puncture-aspiration and a sacrifice of animal. The collected material was processed using a Ficoll-Hypaque density gradient (d = 1.077) according to Boyüm.10 The isolated cells were plated as described below. Assays were performed in 25-cm2
Results
The 2:1 ratio of satellite cells/stem cell was chosen because upon morphological observation the vials were more confluent in this proportion. Ten cocultures were performed, but only four groups (I, II, VII, and IX) were transplanted (Table 1, Table 2).
Discussion
It is concluded that cultivation of two distinct cell groups simultaneously may optimize the results already obtained from the transplantation of individually cultivated SM and MSC. We have demonstrated the possibility of coinjection of cultured cells preceeded by cocultivation may facilitate autologous transplantation, and transplants with this model are ongoing.
References (10)
- et al.
Smooth muscle cell cell transplantation into myocardial scar tissue improves heart function
J Mol Cell Cardiol
(1999) - et al.
Comparison of the effects of fetal cardiomyocyte and skeletal myoblast transplantation on post-infarct left ventricular function
J Thorac Cardiovasc Surg
(2000) - et al.
Fetal cell transplantationa comparison of three cell types
J Thorac Cardiovasc Surg
(1999) - et al.
Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs
Ann Thorac Surg
(2002) - et al.
Comparison between the growth pattern of cell cultures from normal and Duchenne dystrophy muscle
J Neurol Sci
(1984)
Cited by (15)
Immunophenotypic Expression by Flow Cytometric Analysis of Cocultured Skeletal Muscle and Bone Marrow Mesenchymal Stem Cells for Therapy Into Myocardium
2008, Transplantation ProceedingsCitation Excerpt :Collection of skeletal muscle was obtained by biopsy of the posterior tibiae muscle with SMC isolations performed by enzymatic dissociation in accordance with Delaporte et al.12 Collection of MonoSC was obtained by puncture aspiration of bone marrow from the iliac crest with isolation by Ficoll-Hypaque (d = 1.077) density gradient in accordance with Boyüm.13 Coculture of SMC and BMMeSC was established as described by Carvalho et al.14 All cultures started with the same cell concentration (5 × 105/mL) were maintained in an Incubator with 5% CO2 at 37°C in Dulbecco's Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 15% fetal calf serum (FCS; Gibco, United States), 10 μg/mL of insulin growth factor type I (Sigma, St Louis, Mo, United States), 1% antibiotic (100 μg/mL streptomycin–100 μg/mL penicillin), and 10−6 mol/L dexamethasone), which was changed every 48 hours for 21 days. The cell count was performed in a hemocytometer.
Bone marrow-derived stem cell interactions with adult cardiomyocytes and skeletal myoblasts in vitro
2006, Cardiovascular Revascularization MedicineIntraurethral co-transplantation of bone marrow mesenchymal stem cells and muscle-derived cells improves the urethral closure
2018, Stem Cell Research and Therapy