Abstract

Asbestos fibers are well known environmental carcinogen, however, the underlying mechanisms of their action have still not clearly been identified. Asbestos is capable of depleting glutathione and generating reactive oxygen species (ROS), which are important mediators of damage in biological system. Asbestos-induced mutagenecity, may be mediated by the generation. It is known that a number of scavengers and antioxidants attenuate asbestos-induced ROS release. Furthermore, it is known that garlic, contains numerous sulfur compounds and glutathione precursors which act as antioxidants and also demonstrate anticarcinogenic properties. The aim of this study was to investigate whether garlic extract has any influence on asbestos-mediated genotoxicity. As an assay system, we applied the micronucleus assay, sister chromatid exchanges, and chromosomal aberrations with human peripheral blood lymphocytes, which has already been used to analyze the genotoxicity of asbestos fibers. Our results indicate that garlic extract, when administered to the lymphocytes cell culture simultaneously with chrysotile reduced the rates of micronucleus formation, sister chromatid exchanges, and chromosomal aberrations significantly. We conclude that garlic extract may be an efficient, physiologically tolerable quencher of asbestos-mediated genotoxicity.

Keywords: Garlic extract (GE); Reactive oxygen species (ROS); Antioxidant enzyme (AOE); Dimethylthiourea (DMTU); Superoxide dismutase (SOD); Human peripheral blood lymphocytes (HUPBL); Diallyl sulfide (DAS); Diallyl disulfide (DADS); Glutathione S-transferase (GST)

Article Outline

1. Introduction

2. Materials and methods

2.1. Chemicals

2.2. Asbestos fibers

2.3. Garlic preparation

2.4. HUPBL culture and treatment conditions

2.5. Micronucleus assay (MN)

2.6. Sister chromatid exchanges (SCE)

2.7. Chromosomal aberrations (CA)

2.8. Statistical analysis

3. Results

4. Discussion

Acknowledgements

References