Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas)☆
Section snippets
Description of B. reuteri
B. reuteri (reu’te.ri. N.L. masc. gen. n. reuteri, of Reuter, named after Professor Gerhard Reuter, in honour of his research on intestinal microbiota).
Cells are Gram-positive, irregularly shaped rods with rounded or tapered end, usually swollen and branched, measuring 0.3–1.0 × 1.5–6 μm. Cells are non-motile and usually occur singly or in pairs. Facultatively anaerobic, catalase-negative and F6PPK-positive. Colonies on MRS agar supplemented with 50 mg (l−1) l-cysteine after incubation for two days
Description of B. callitrichos
B. callitrichos (cal.li’tri.chos. N.L. n. callithrix-trichos, scientific name of a genus of primates; N.L. gen. n. callitrichos, of Callithrix, isolated from C. jacchus).
Cells are Gram-positive, irregularly shaped rods with rounded or tapered end, usually swollen and branched, measuring 0.6–1.2 × 3–8 μm. Cells are non-motile and usually occur singly, in pairs or in chains. Facultatively anaerobic, catalase-negative and F6PPK-positive. Colonies on MRS agar supplemented with 50 mg (l−1) l-cysteine
Description of B. saguini
B. saguini (sa’gui.ni. N.L. gen. n. saguini, of Saguinus, isolated from S. midas).
Cells are Gram-positive, irregularly shaped rods with rounded or tapered end, usually swollen and branched, measuring 0.4–1.0 × 2–6 μm. Cells are non-motile and usually occur singly, in pairs or in chains. Facultatively anaerobic, catalase-negative and F6PPK-positive. Colonies on MRS agar supplemented with 50 mg (l−1) l-cysteine after incubation for two days are beige, smooth and approximately 1–2 mm in diameter under
Description of B. stellenboschense
B. stellenboschense (stel.len.bos.chen'se. N.L. neut. adj. stellenboschense, of Stellenbosch, a town in South Africa where the type strain was isolated).
Cells are Gram-positive, irregularly shaped rods, usually swollen and branched, measuring 0.5–1.0 × 4–10 μm. Cells are non-motile and usually occur singly, in pairs or in chains. Facultatively anaerobic, catalase-negative and F6PPK-positive. Colonies on MRS agar supplemented with 50 mg (l−1) l-cysteine after incubation for two days are white,
Description of B. biavatii
B. biavatii (bi.a.va’ti.i. N.L. gen. masc. n. biavatii, of Biavati, named after Professor Bruno Biavati, in honour of his research on bifidobacteria).
Cells are Gram-positive or -variable, irregularly shaped rods, usually swollen and branched, measuring 0.5–1.0 × 3–8 μm. Cells are non-motile and usually occur singly, in pairs or in chains. Facultatively anaerobic, catalase-negative and F6PPK-positive. Colonies on MRS agar supplemented with 50 mg (l−1) l-cysteine after incubation for two days are
Acknowledgements
We are grateful to J.P. Euzéby (Société de Bactériologie Systématique et Vétérinaire and École Nationale Vétérinaire de Toulouse) for advice on the naming of novel species and to Mrs. Birgit Grün (DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) for her excellent technical assistance with the determination of G + C contents in our novel strains. Thanks are also due to the World of Birds (Cape Town, South Africa) for their assistance in collecting samples. A. Endo received a
References (23)
- et al.
A rapid method for the base ratio determination of bacterial DNA
Anal. Biochem.
(1977) - et al.
Diversity of Lactobacillus and Bifidobacterium in feces of herbivores, omnivores and carnivores
Anaerobe
(2010) - et al.
Ecology of the bifidobacteria
Am. J. Clin. Nutr.
(1977) - et al.
Modification of the phosphoketolase assay for rapid identification of bifidobacteria
J. Microbiol. Methods
(2000) - et al.
Determination of DNA base composition by reversed-phase high-performance liquid chromatography
FEMS Microbiol. Lett.
(1984) - et al.
Bifidobacterium ruminantium sp. nov. and Bifidobacterium merycicum sp. nov. from the rumens of cattle
Int. J. Syst. Bacteriol.
(1991) - et al.
Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials
Int. J. Syst. Evol. Microbiol.
(2005) - et al.
Oenococcus kitaharae sp. nov., a non-acidophilic non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue
Int. J. Syst. Evol. Microbiol.
(2006) - et al.
Bifidobacterium scardovii sp. nov., from human sources
Int. J. Syst. Evol. Microbiol.
(2002) - et al.
New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences
Int. J. Syst. Evol. Microbiol.
(2001)
Bifidobacterium bombi sp. nov., from the bumblebee digestive tract
Int. J. Syst. Evol. Microbiol.
Cited by (0)
- ☆
The GenBank/EMBL/DDBJ accession numbers for 16S rRNA gene sequences, dnaJ1 sequences and hsp60 sequences of B. reuteri AFB22-1T, B. callitrichos AFB22-5T, B. saguini AFB23-1T, B. stellenboschense AFB23-3T and B. biavatii AFB23-4T are AB613259 and AB559503–AB559506, AB613260–AB613264 and AB674318–AB674322, respectively.