ScienceDirect - Systematic and Applied Microbiology : Azorhizobium doebereinerae sp. Nov. Microsymbiont of Sesbania virgata (Caz.) Pers.

Systematic and Applied Microbiology
Volume 29, Issue 3, 18 April 2006, Pages 197-206

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doi:10.1016/j.syapm.2005.09.004

Azorhizobium doebereinerae sp. Nov. Microsymbiont of Sesbania virgata (Caz.) Pers.

Fátima Maria de Souza Moreira^a^,^,, Leonardo Cruz^b, Sérgio Miana de Faria^c, Terence Marsh^d, Esperanza Martínez-Romero^e, Fábio de Oliveira Pedrosa^b, Rosa Maria Pitard^c and J. Peter W. Young^f

^aDepto. Ciência do solo, Universidade Federal de Lavras, C.P. 3037 , 37 200–000, Lavras, MG, Brazil ^bUniversidade Federal do Paraná, C.P. 19046, 81513-990, PR, Brazil ^cEmbrapa Agrobiologia, antiga estrada Rio, São Paulo km 47, 23 851-970, Seropédica, RJ, Brazil ^dCenter for Microbial Ecology, Michigan State University, MI 48824, USA ^eCentro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de Mexico, Apdo Postal 565-A, Cuernavaca, Mor, México ^fDepartment of Biology, University of York, PO Box 373, York YO10 5YW, UK

Received 18 August 2005.

Available online 17 October 2005.

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Abstract

Thirty-four rhizobium strains were isolated from root nodules of the fast-growing woody native species Sesbania virgata in different regions of southeast Brazil (Minas Gerais and Rio de Janeiro States). These isolates had cultural characteristics on YMA quite similar to Azorhizobium caulinodans (alkalinization, scant extracellular polysaccharide production, fast or intermediate growth rate). They exhibited a high similarity of phenotypic and genotypic characteristics among themselves and to a lesser extent with A. caulinodans. DNA:DNA hybridization and 16SrRNA sequences support their inclusion in the genus Azorhizobium, but not in the species A. caulinodans. The name A. doebereinerae is proposed, with isolate UFLA1-100 (=BR5401, =LMG9993=SEMIA 6401) as the type strain.

Keywords: Taxonomy; Tropical legume; Specificity; Root nodules; Nitrogen-fixing species

Article Outline

Introduction
Materials and methods Strains Phenotypic characteristics Cell characteristics DNA extraction REP-PCR and cluster analysis Multilocus enzyme electrophoresis (MLEE) Polyacrylamide gel electrophoresis of total bacterial proteins 16S rDNA sequencing DNA–DNA hybridization Phylogenetic analysis
Results and discussion Utilization of carbon sources Growth and nitrogenase activity under free-living conditions and on LB medium REP-PCR and cluster analysis Multilocus enzyme electrophoresis (MLEE) Polyacrylamide gel electrophoresis of total bacterial proteins 16S rDNA sequencing DNA–DNA hybridization Cell characteristics Description of Azorhizobium doebereinerae [doe.ber.ein’erae, N.L. gen. n. doebereinerae, named in honour of Johanna Döbereiner, an outstanding Brazilian microbiologist (1924–2000)]
Acknowledgements
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Systematic and Applied Microbiology
Volume 29, Issue 3, 18 April 2006, Pages 197-206

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