Infection/InflammationRemifentanil preconditioning protects the small intestine against ischemia/reperfusion injury via intestinal δ- and μ-opioid receptors
Section snippets
Animals and operative procedure
The use of animals in this study was approved by the Animal Care Committee at Sun Yat-sen University (Guangzhou, China), and all animal experiments were conducted in accordance with the guidelines of the National Institutes of Health.
Adult, male, Sprague-Dawley rats weighing 230–280 g were used. The rats were housed in the departmental animal facility under normal (12 hours on/12 hours off) light/dark cycles and fasted overnight before the study but had free access to water. All animals were
Experiment 1: In vivo study
All animals survived the experimental period. There were no statistical differences in body weight or body temperature during the experiments among the groups (data not shown, P > .1).
Discussion
In the current study, we have shown that remifentanil preconditioning could improve intestinal morphologic changes, inhibits epithelial cell apoptosis, and confers intestinal protection without altering the hemodynamics and blood gas analysis in an in vivo rat model of I/R. Similarly in the IEC-6 cell culture model of OGD/R, remifentanil preconditioning increased IEC-6 cell viability as well as decreased the cell apoptosis after 4 hours of OGD followed by 4 hours of reintroduction of oxygen to
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2021, Biomedicine and PharmacotherapyCitation Excerpt :In one study, after the clamp was released, the author added 3 drops of lidocaine on SMA to relieve spasm of SMA, facilitating reperfusion [35]. Ischemia time varied from 30 to 60 min and reperfusion time from 15 to 240 min [1,5,12,17–19,21–28,31–41,43–47,49–51,53,55–58,60–66], the most frequently used ischemia and reperfusion time was 60 min ischemia with 120 min reperfusion. However, 4 researchers applied the reperfusion time up to 24 h [19,46,47,59].
Microarray Profiling and Functional Identification of LncRNA in Mice Intestinal Mucosa Following Intestinal Ischemia/Reperfusion
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2018, PeptidesCitation Excerpt :Remifentanil-induced preconditioning exhibited cross-talk with A1 and A2B adenosine receptors in ischemic-reperfused rat heart [590]. Remifentanil preconditioning protected the small intestine against ischemia/reperfusion injury through intestinal delta and mu opioid receptors [935]. Delta opioid receptor activation increased mRNA expression in the rat heart under prolonged hypoxia [1181].
Propofol-based anaesthesia versus sevoflurane-based anaesthesia for living donor kidney transplantation: results of the VAPOR-1 randomized controlled trial
2017, British Journal of AnaesthesiaCitation Excerpt :The doses used in these experiments are rather high (heart, 6.0 μg kg−1 min−1) compared with the continuous dose we used in our clinical setting (range 0.08–0.12 μg kg−1 min−1).33 One study using lower doses (0.1–1 μg kg−1 min−1) for preconditioning of the intestine reported a dose-independent effect.34 Studies using continuous or semi-continuous infusion during the entire procedure or before and during the ischaemic period show a dose-independent effect in the liver (dose ranging from 0.4 to 10.0 μg kg−1 min−1), but in the brain a protective effect was seen only in the high range dose (1.8 μg kg−1 min−1).3536