Elsevier

Stem Cell Research

Volume 60, April 2022, 102729
Stem Cell Research

Lab Resource: Single Cell Line
Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology

https://doi.org/10.1016/j.scr.2022.102729Get rights and content
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Abstract

The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated with tooth agenesis, orofacial clefting, and nail dysplasia. Here, we generated a MSX1 knockout cell line from human embryonic stem cell (hESC) line (H9) by CRISPR/cas9-mediated gene targeting. This cell line may serve as a valuable in vitro cell model for MSX1 mutation-related diseases and help to gain more insight into the biological function of MSX1.

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