Elsevier

Research in Microbiology

Volume 164, Issue 2, February–March 2013, Pages 155-163
Research in Microbiology

Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W

https://doi.org/10.1016/j.resmic.2012.11.003Get rights and content
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Abstract

Utilization of an enrichment technique led to isolation of a bacterium from municipal waste-contaminated soil in which acenaphthene was used as the sole source of carbon and energy. The isolate was identified as Acinetobacter sp. strain AGAT-W based on morphological, nutritional and biochemical characteristics and 16S rRNA sequence analysis. Characterization of metabolites by HPLC and GC–MS suggested hydroxylation of acenaphthene to 1-acenaphthenol, which was subsequently transformed to catechol via acenaphthenequinone, naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid and salicylic acid before entering into the tricarboxylic acid cycle. Detection of key enzymes, viz., 1-acenaphthenol dehydrogenase, salicylaldehyde dehydrogenase and catechol 1,2-dioxygenase, in the cell-free extract of Acinetobacter sp. further supported the proposed degradation pathway. This study proposes a metabolic pathway involved in acenaphthene assimilation in strain AGAT-W.

Keywords

Polycyclic aromatic hydrocarbon
Acenaphthene
Acinetobacter
Biodegradation
Metabolic pathway

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