Original ArticleMethylation-associated silencing of miR-193b improves the radiotherapy sensitivity of esophageal cancer cells by targeting cyclin D1 in areas with zinc deficiency
Section snippets
Materials and methods
This study was approved by the Institutional Human Ethics Committee of Hebei Medical University Fourth Hospital, and prior informed consent was obtained from all the patients.
Results
To investigate the radioresistance mechanisms of EC cells, we first compared the changes that occurred in radioresistant cell lines. In KYSE150R cells, the ratio of G0/G1 phase increased significantly compared with KYSE150 (68% vs 50%). The cell cycle of the radioresistant cells was blocked at the G0/G1 phase (Supplementary Fig. 2B). So we tested the expression level of Cyclin D1, a key protein that promotes the cell cycle from G1 to S phase, in KYSE150R and KYSE150 cells. The results showed
Discussion
Radiotherapy is an important treatment for EC, but radioresistance significantly restricts the success of radiotherapy in the treatment of EC [15]. The efficacy of radiotherapy is limited by the selection of a radiation dose that could injury cancer tissues without damaging normal tissues. Radioresistance could be induced by radiation during radiotherapy treatment. The acquisition of radioresistance in malignant tumor cells remains the major cause of failure in the treatment of patients with
Acknowledgements
This study was supported by grants from the National Natural Scientific Foundation of China (81871922) and the Hebei Province Health Department (20170745).
Conflicts of interest statement
No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication.
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