Comparative proteomic responses of two bermudagrass (Cynodon dactylon (L). Pers.) varieties contrasting in drought stress resistance

doi:10.1016/j.plaphy.2014.06.006

Plant Physiology and Biochemistry

Volume 82, September 2014, Pages 218-228

https://doi.org/10.1016/j.plaphy.2014.06.006 Get rights and content

Highlights

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Different organs of bermudagrass show natural variation of drought resistance.
•
Proteomic analysis was performed in leaves and stems of bermudagrass.
•
Drought-responsive proteins were identified in two bermudagrass varieties.

Abstract

Drought (water-deficit) stress is a serious environmental problem in plant growth and cultivation. As one of widely cultivated warm-season turfgrass, bermudagrass (Cynodon dactylon (L). Pers.) exhibits drastic natural variation in the drought stress resistance in leaves and stems of different varieties. In this study, proteomic analysis was performed to identify drought-responsive proteins in both leaves and stems of two bermudagrass varieties contrasting in drought stress resistance, including drought sensitive variety (Yukon) and drought tolerant variety (Tifgreen). Through comparative proteomic analysis, 39 proteins with significantly changed abundance were identified, including 3 commonly increased and 2 decreased proteins by drought stress in leaves and stems of Yukon and Tifgreen varieties, 2 differentially regulated proteins in leaves and stems of two varieties after drought treatment, 23 proteins increased by drought stress in Yukon variety and constitutively expressed in Tifgreen variety, and other 3 differentially expressed proteins under control and drought stress conditions. Among them, proteins involved in photosynthesis (PS), glycolysis, N-metabolism, tricarboxylicacid (TCA) and redox pathways were largely enriched, which might be contributed to the natural variation of drought resistance between Yukon and Tifgreen varieties. These studies provide new insights to understand the molecular mechanism underlying bermudagrass response to drought stress.

Introduction

Drought (water-deficit) stress is one of the major environmental stresses that seriously limits plant distribution, growth and yield worldwide (Shi et al., 2012, Shi et al., 2013a, Shi et al., 2013b, Shi et al., 2014, Zhao et al., 2011). With the changes of environment and global climate, drought stress not only seriously affects plant growth and development, but also breaks the ecological balance of the ecosystem (Shi et al., 2012, Shi et al., 2013a, Shi et al., 2013b, Shi et al., 2014, Zhao et al., 2011).

Bermudagrass (Cynodon dactylon (L). Pers.) is one of wildly cultivated warm-season turfgrass on lawns, parks and sport fields, with drastic natural variation in the drought stress resistance among different varieties (Shi et al., 2012, Zhao et al., 2011). Recently, we identified three groups of bermudagrass that differed in drought resistance, including drought sensitive variety (Yukon), moderately tolerant variety (SR9554) and drought tolerant variety (Tifgreen) (Shi et al., 2012). Comparative physiological analysis among these varieties suggested that changes of water status, osmolyte accumulation and antioxidant defense system during drought stress might contribute to the natural variation of bermudagrass drought resistance (Shi et al., 2012). However, the molecular mechanisms underlying bermudagrass response to drought stress remain largely unknown. Recent studies in plant proteome have made it powerful and effective approach to identify proteins that were essential of stress response (Komatsu et al., 2012, Mohammadi et al., 2012, Shi et al., 2013a, Xu and Huang, 2010, Zhao et al., 2011). Currently, the drastic natural variations among different bermudagrass varieties provide effective approach to investigate the molecular, genetic, proteomic, and metabolic mechanisms during drought stress response (Mohammadi et al., 2012, Shi et al., 2012, Xu and Huang, 2010, Zhao et al., 2011). Bermudagrass grows and reproduces rapidly through propagation by both seeds and cutting branches (Shi et al., 2012, Shi et al., 2013a, Shi et al., 2013b). When grown under drought stress condition, the leaves of bermudagrass turned yellow and brown or died off, but the branch and rhizome were still alive (Shi et al., 2012, Shi et al., 2013a, Shi et al., 2013b).

Bermudagrass adapts to different cultivation conditions all over the world, however, research about bermudagrass stress response is limited because of non-availability of genome information. Most research groups mainly paid attention to physiological level changes and focused on the leave samples (Hu et al., 2009, Shi et al., 2012, Shi et al., 2013a, Shi et al., 2013b, Xu and Huang, 2010, Zhao et al., 2011). In this study, we identified 39 drought-responsive proteins in both leaves and stems of two bermudagrass varieties contrasting in drought stress resistance using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF–MS). Among these proteins, 23 proteins were up-regulated by drought stress in leaves or stems of Yukon variety and were constitutive expressed in Tifgreen variety. Further cluster and pathway analyses provided some new insights to understand the molecular mechanism underlying bermudagrass response to drought stress.

Section snippets

Plant materials and growth conditions

Two bermudagrass varieties of Yukon [provided by American Seed Research of Oregon Company (http://www.sroseed.com/)] and Tifgreen [provided by Wuhan Green Garden Turfgrass Company (http://www.whcp66.com/)http://www.whcp66.com/)] were used in this study. The healthy cutting shoot-tips from these two bermudagrass varieties were cultured in the soil in the growth room as previously described (Shi et al., 2012), which was controlled at an irradiance of about 150 μmol quanta m⁻² s⁻¹, 25 ± 2 °C,

Comparison of drought-triggered cell damage in leaves and stems of Yukon and Tifgreen varieties

Under control condition, no significant differences were obtained in plant growth and cell damage (leaves and stems) between Yukon and Tifgreen varieties (Fig. 1A–C). When subjected to drought stress condition, plant growth was inhibited and EL in both of leaves and stems gradually increased in both bermudagrass varieties (Fig. 1A–C). However, Tifgreen variety showed better growth than Yukon variety under drought stress condition, with lower EL in both of leaves and stems (Fig. 1A–C).

Discussion

In recent years, comparative physiological, transcriptomic, proteomic and metabolomic studies are the most important and effective strategies to reveal physiological and molecular mechanisms of plant genotypes with contrasting developmental phenotypes or stress resistance (Mohammadi et al., 2012, Shi et al., 2012, Shi et al., 2013a, Shi et al., 2014, Xu and Huang, 2010, Zhao et al., 2011). In the previous research, we found that the changes of water status, osmolyte accumulation and antioxidant

Conclusion

Taken together, this study represents the first proteomic analysis in both leaves and stems of two bermudagrass varieties contrasting in drought stress resistance. Notably, 74% of 39 identified proteins exhibited higher protein abundance in the drought-tolerant variety under control and drought stress conditions, while 62% of 39 identified proteins were induced in the drought-sensitive variety under drought stress and were constitutively expressed in the drought-tolerant variety. These

Acknowledgements

We thank American Seed Research of Oregon Company (http://www.sroseed.com/) and Wuhan Green Garden Turfgrass Company (http://www.whcp66.com/)http://www.whcp66.com/) for providing the materials of Yukon and Tifgreen varieties, respectively. We also thank Prof. Pingfang Yang, Dr. Ming Li and Dr. Xiaojian Yin for their help in the proteomic research. This research was supported by "the Hundred Talents Program”, and the Knowledge Innovative Key Program of Chinese Academy of Sciences (No.

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¹: These authors contributed equally to this study.

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Research articleComparative proteomic responses of two bermudagrass (Cynodon dactylon (L). Pers.) varieties contrasting in drought stress resistance

Highlights

Abstract

Introduction

Section snippets

Plant materials and growth conditions

Comparison of drought-triggered cell damage in leaves and stems of Yukon and Tifgreen varieties

Discussion

Conclusion

Acknowledgements

Plant Physiol. Biochem

Plant Physiol. Biochem

Curr. Opin. Plant Biol.

J. Plant Physiol.

Plant Physiol. Biochem

J. Plant Physiol.

A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding

Anal. Biochem

Expression of the maize ZmGF14-6 gene in rice confers tolerance to drought stress while enhancing susceptibility to pathogen infection

J. Exp. Bot.

A nuclear-located HSP70 confers thermoprotective activity and drought stress tolerance on plants

Biotechnol. Lett.

Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants

Plant Cell. Rep.

High potential iron-sulfur proteins and their role as soluble electron carriers in bacterial photosynthesis: tale of a discovery

Photosynth. Res.

Transgenic expression of plant clitinases to enhance disease resistance

Biotechnol. Lett.

Open source clustering software

Bioinformatics

Rubiscolytics: fate of Rubisco after its enzymatic function in a cell is terminated

J. Exp. Bot.

Cytosolic glyceraldehyde-3-phosphate dehydrogenases interact with phospholipase Dδ to transduce hydrogen peroxide signals in the Arabidopsis response to stress

Plant Cell.

Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates 14-3-3 protein to confer drought tolerance in rice seedlings

Plant Mol. Biol.

Differential accumulation of dehydrins in response to water stress for hybrid and common bermudagrass genotypes differing in drought tolerance

J. Plant Physiol.

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Funct. Integr. Genomics

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J. Proteome Res.

Research article
Comparative proteomic responses of two bermudagrass (Cynodon dactylon (L). Pers.) varieties contrasting in drought stress resistance