Abstract
Purchase PDF (226 K)
E-mail Article
Add to my Quick Links
Cited By in Scopus (3)
Purchase PDF (81 K)
doi:10.1016/j.phytochem.2006.07.019 
Copyright © 2006 Elsevier Ltd All rights reserved.
The rolC gene induces expression of a pathogenesis-related β-1,3-glucanase in transformed ginseng cells
Konstantin V. Kiseleva, c, Mikhail I. Kusaykinb, Alexandra S. Dubrovinaa, c, Denis A. Bezverbnya, c, Tatiana N. Zvyagintsevab and Victor P. Bulgakova, 
, 
Received 9 September 2005;
References and further reading may be available for this article. To view references and further reading you must purchase this article.
Abstract
The Agrobacterium rhizogenes rolC oncogene is capable of stimulating production of secondary metabolites in transformed plant cells that suggest its possible involvement in plant defense reactions. We tested whether the gene could also affect production of pathogenesis-related proteins. Using a well-known group of PR-proteins, such as β-1,3-glucanases, we observed a 10-fold increase in total β-1,3-glucanase activity in rolC-transformed Panax ginseng cells compared with normal cells. The increase was due to the production of a salicylic acid-activated β-1,3-glucanase isoform. We isolated cDNA of the corresponding β-1,3-glucanase gene (Pg-glu1), which shared 38–60% sequence identity with previously reported sequences of plant β-1,3-glucanases at the protein level. Levels of Pg-glu1 mRNA transcripts were tightly correlated with expression of the rolC gene. Our data, together with previously reported information, indicate that A. rhizogenes can activate plant defense reactions via expression of T-DNA oncogenes.
Graphical abstract
In ginseng cells, the rolC gene activates expression of β-1,3-glucanase, indicating that Agrobacterium rhizogenes has a potential to activate plant defense.
Keywords: Panax ginseng; rolC gene; Pg-glu1; β-1,3-Glucanase; PR-proteins
Abbreviations: SA, salicylic acid; MeJA, methyl jasmonate
Article Outline
- 1. Introduction
- 2. Results
- 2.1. Transformed P. ginseng tissues and parameters of rolC gene expression
- 2.2. Molecular analysis of the ginseng β-1,3-glucanase gene
- 2.3. Expression of rolC in transformed cells coincides with Pg-glu1 expression and a corresponding increase in β-1,3-glucanase activity
- 2.4. Pg-glu1 encodes a SA-dependent but MeJA-independent glucanase
- 3. Discussion
- 4. Experimental
- 4.1. Chemicals
- 4.2. Cell cultures and media
- 4.3. Activity and gel-permeation chromatography of β-1,3-glucanases
- 4.4. Semiquantitative RT-PCR
- 4.5. mRNA quantification
- 4.6. Isolation and sequence analysis of Pg-glu1
- Acknowledgements
- Appendix A. Supplementary data
- References
