Fig. 1. Effect of PQ addition on the activity of extracellular laccase in T. versicolor (A) and A. biennis (B) cultures grown on liquid mineral medium. PQ was applied to 10-day-old cultures at 25 μM (T. versicolor) or 20 μM (A. biennis). Medium samples were collected up to 120 h after PQ treatment. Data are mean ± SD from at least three independent experiments performed in duplicate.

Fig. 2. Native PAGE electrophoresis of extracellular laccase from T. versicolor and A. biennis (collected for 120 h after PQ addition). Samples of ultrafiltration concentrated culture fluid, 25 μl were loaded in each lane. Lane 1, samples of control A. biennis cultures; lane 2, samples of PQ-treated A. biennis cultures; lane 3, samples of T. versicolor control cultures; and lane 4, samples of PQ-treated T. versicolor. Activity staining was obtained with guaiacol.

Fig. 3. Changes in the extracellular superoxide anion radicals level (A) and intracellular level of formaldehyde (B) measured for 72 h after PQ-treatment in T. versicolor and A. biennis cultures. The relative level of SOR and the concentration of FA were expressed as a percentage of control values at the beginning of experiment (just before PQ addition). Data represents the mean of triplicate measurements. Standard deviations were less than 8% (for SOR level) and less than 5% (for FA) of the mean values.

Fig. 4. Effect of PQ application to 10-day-old T. versicolor and A. biennis cultures on activities of extracellular superoxide dismutase (A and B) and intracellular catalase (C and D) measured for 120 h after treatment. Data are means ± SD from at least three independent experiments performed in duplicate.

Fig. 5. Effect of PQ application to 10-day-old T. versicolor and A. biennis cultures on dry weight of the mycelia estimated for 120 h after treatment. Data are means ± SD from at least three independent experiments performed in duplicate (SD not exceed 3%).

Activities of extra- and intracellular Mn peroxidase (MnP) (nkat/mg protein) in the T. versicolor and A. biennis PQ- treated cultures

Note. The 10-day-old fungal cultures were treated with PQ water solution. The mycelia as well as the culture medium fluid were collected for 120 h after PQ addition. Data are means ± SD from at least three independent experiments performed in duplicate.

Changes of glutathione (GSH) concentration in the T. versicolor and A. biennis PQ-treated mycelia

Note. Data are means ± SD from at least three independent experiments performed in duplicate.

Extracellular phenolic compounds concentration (μg/ml) in T. versicolor and A. biennis PQ-treated cultures

Note. The concentration of phenolic compounds was measured at 24, 72, and 120 h after PQ addition using SA as a reaction substrate. Data are means ± SD from at least three independent experiments performed in duplicate.