Fig. 1. The effect of icv enterostatin (1 nmole) on food intake in wild-type and MC4R −/− mice. Data represent means ± S.E.M. for five to eight animals per group. ^*p < 0.01 compared to saline group.

Fig. 2. The effect of SHU9119 on the feeding response to enterostatin in rats adapted to high fat diets. Data represent means ± S.E.M. for 2 h food intake for five to eight animals per group. ^*p < 0.01 compared to saline group.

Fig. 3. Colocalization of c-fos and αMSH immunoreactivity in the arcuate nucleus after enterostatin injections into the amygdala. (A) After saline injection, white arrows sshowing α-MSH positive cells; (B) after enterostatin injection into the amygdala, black arrows showing fos nuclear staining in α-MSH positive cells.

Fig. 4. The effect of enterostatin on gene expression of AgRP and POMC in amygdala (1 nmole enterostatin onto amygdala) and hypothalamus (1 nmole enterostatin icv) of rats adapted to high fat diets and in GT1-7 cells in culture (10–1000 nM enterostatin). ^*p < 0.01 compared to vehicle group.

c-Fos expression in αMSH neurons of the arcuate nucleus in rats receiving enterostatin into the amygdala

Values represent means ± S.E.M. of identified neurons in the arcuate nucleus for four rats in each group. Two represenative sections from each rat were counted.