Fig. 1. Schematic of the first step of peptidoglycan biosynthesis where MurA, a UDP-N-acetylglucosamine enolpyruvyl transferase, catalyzes the enolpyruvyl transfer reaction from enolpyruvate to uridine 5′-diphospho-N-acetylglucosamine.

Fig. 2. Consensus peptide sequences obtained from screening the PH.D.-C-7-C and 12-mers phage-encoded peptide libraries against MurA. The data were compiled from the sequencing of 60 eluted phages after the fourth round of competitive biopanning. The two peptides synthesized PEP 1354 and PEP 1361 and containing consensus amino acid sequences are shown below the alignment. Acidic amino acids (D, E) are in green, polar amino acids (Q, N) are in light green, basic amino acids (K, R, H) are in blue, hydrophobic amino acids(I, L, M, V) are in pink, hydrophobic aromatic amino acids (F, Y, W) are in red, small amino acids (A, S, C, T) are in pink, G (tiny amino acid) is in orange and P (leading to turn formation is in black (classified according the Venn diagram for defining the relationships between amino acids). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)

Fig. 3. IC₅₀ determination for the PEP 1354 peptide inhibitor of MurA ATPase activity.

Phage titers after four rounds of biopanning using MurA and the C-7-C and 12-mers phage peptide libraries

Frequency of recovery and ELISA relative affinity ratio of selected peptide sequences