Original ArticleUsefulness of PAX8 Immunohistochemistry in Adult Intraocular Tumor Diagnosis
Section snippets
Methods
Informed consent was obtained from patients 6, 7, and 8 (Wills Eye Hospital). For patients 1 through 5 and 9 through 34 (Royal Hallamshire Hospital, United Kingdom), ethics committee approval was obtained (study approval nationally; identifier, 15/NW/0239) from the Sheffield Teaching Hospitals Research & Development Office (study no., STH 21118; substudy to study no., STH15427) according to the United Kingdom Human Tissue Act guidelines that permit the use of retrospective, archived human
Results
PAX8 staining was interpreted as positive if it stained the nucleus only. Very little variation was found in the density of the nuclear staining; when positive, it was invariably intensely brown. Some cases showed a blush of nonspecific cytoplasmic staining. Table 1 summarizes the key patient clinical information and the histopathologic details of the tumors.
Discussion
This study mapped the expression of PAX8 protein in normal ocular structures and showed its expression in a select number of intraocular tumors. The expression of PAX8 in iris and ciliary body epithelium, corneal and lens epithelium, and some retinal neurons suggests that PAX8 may be involved in their homeostasis and regeneration.
Observation of PAX8 expression in normal iris pigment epithelium and ciliary body epithelium led to a prediction that tumors arising from these structures may express
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See Commentary on page 779.
Disclosure(s): All authors have completed and submitted the ICMJE disclosures form. The author(s) have no proprietary or commercial interest in any materials discussed in this article.
Supported by Sheffield Teaching Hospitals Charity Small Grants Fund (grant no.: 340619); and the Pennsylvania Lions Sight Conservation and Eye Research Foundation, Inc., Beaver Falls, Pennsylvania. The funding organizations had no role in the design or conduct of this research.
HUMAN SUBJECTS: Human subjects were included in this study. The human ethics committees at Wills Eye Hospital and Sheffield Teaching Hospitals Research & Development Office approved the study. All research adhered to the tenets of the Declaration of Helsinki. Informed consent was obtained from patients 6, 7, and 8 (Wills Eye Hospital); for patients 1 through 5 and 9 through 34 (Royal Hallamshire Hospital, United Kingdom), ethics committee approval was obtained (study approval nationally; identifier, 15/NW/0239) from the Sheffield Teaching Hospitals Research & Development Office (study no., STH 21118; substudy to study no., STH15427) according to the United Kingdom Human Tissue Act guidelines that permit the use of retrospective, archived human biopsy material surplus to diagnosis, through block consent from the research office.
No animal subjects were included in this study.
Author Contributions:
Conception and design: Mudhar, Sanderson, Pheasey
Analysis and interpretation: Mudhar, Milman, Eagle, Sanderson, Pheasey, Paine, Salvi, Rennie, Rundle, C.Shields, J.A.Shields
Data collection: Mudhar, Milman, Eagle, Sanderson, Pheasey, Paine, Salvi, Rennie, Rundle, C.Shields, J.A.Shields
Obtained funding: N/A
Overall responsibility: Mudhar, Milman, Salvi, Rennie, Rundle, C.Shields