Original articleRole of Soluble Vascular Endothelial Growth Factor Receptor-1 in the Vitreous in Proliferative Diabetic Retinopathy
Section snippets
Materials
Enzyme-linked immunosorbent assay (ELISA) kits for human sVEGFR-1 (Quantikine Human soluble VEGF R1/Flt-1 Immunoassay), human VEGF (Endogen Human VEGF), and PEDF (ChemiKine PEDF) were purchased from R&D Systems (Minneapolis, MN), Pierce Biotechnology Inc (Rockford, IL), and Chemicon (Temecula, CA), respectively. sVEGFR-1 and angiogenesis kits were purchased from Fitzgerald (Concord, MA) and Kurabo (Osaka, Japan), respectively.
Preparation of Vitreous Fluid Samples
This study was conducted according to the tenets of the Declaration
sVEGFR-1, VEGF, and PEDF levels in Vitreous Fluids of Patients with MH or PDR
We measured the concentrations of sVEGFR-1, VEGF, and PEDF in vitreous fluids obtained from patients with MH or PDR. The concentration of sVEGFR-1 was significantly higher in patients with PDR (3949.4±608.9 pg/mL, n = 27) than in patients with MH (1568.8±595.0 pg/mL, n = 12) (P = 0.009) (Fig 1A), as was that of VEGF (PDR, 1316.2±404.6 pg/mL [n = 27]; MH, 11.7±8.1 pg/mL [n = 12]) (P = 0.003) (Fig 1B). For statistical analysis, any VEGF level below the limit of detection was set to zero. Next, we
Discussion
In the present study, we investigated the level of sVEGFR-1 in vitreous fluid samples taken from patients with PDR or MH. Our first finding was that the vitreous concentration of sVEGFR-1 was significantly higher in eyes with PDR than in those with MH. We also found a significantly positive correlation between the sVEGFR-1 and VEGF concentrations in vitreous fluids from patients with PDR. In the vitreous fluid of PDR eyes, our results indicate that the higher level of VEGF may lead to an
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Manuscript no. 2008-293.
Financial Disclosure(s): The authors have no proprietary or commercial interests in any materials discussed in this article.