Systems NeuroscienceResearch PaperPatterns of neurotransmitter receptor distributions following cortical spreading depression
Section snippets
Slice preparation
Adult Wistar rats (250–300 g) were decapitated under deep methohexital anaesthesia and the brains were rapidly removed to ice-cold (4 °C) artificial cerebrospinal fluid (ACSF). The cerebellum was removed and a cut was made to divide the two cerebral hemispheres. Combined striatum-hippocampus–cortex slices containing the temporal cortex, the entorhinal cortex, the subiculum, the dentate gyrus, the hippocampus, as well as the striatum (500 μm) were cut in a nearly horizontal plane. Up to two
Electrophysiological recordings
Cortical SD induced in layer II–III of the temporal neocortex by local application of KCl in 48 slices. The pattern of SD propagation in combined striatum-hippocampus–cortex slices was analysed. Seventy-four percent of KCl induced SD propagated through temporal neocortex (amplitude of 12±1.5 mV and duration of 106±4 s) and ceased in entorhinal cortex (amplitude of 10±1.3 mV and duration of 96±4 s) without any penetration to subcortical regions (abortive SD; Fig. 1,n=37). About 25% of
Discussion
We have assessed 16 transmitter receptors in combined striatum-hippocampus–cortex slices after induction of cortical SD. The present study of receptor changes was intended to provide insights into the regulation of multiple receptor binding sites induced by cortical SD in order to identify its direct effects in the neocortex as well as remote effects in the hippocampus and striatum after 1 h of SD induction. The present data indicate increases in glutamate NMDA, AMPA and kainate receptor
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