Systems NeuroscienceResearch PaperPentylenetetrazole-induced seizures affect binding site densities for GABA, glutamate and adenosine receptors in the rat brain
Section snippets
Animals
We used 8-week-old male Wistar rats (220–250 g body weight, Moellegaard Breeding Centre GmbH, Germany) for all experiments, and animals were housed under standard conditions as previously described (Rauca et al 2004, Bidmon et al 2005). All experimental procedures were conducted according to the institutional guidelines for the use of laboratory animals, the German Animal Welfare Act and approved by the responsible governmental agency.
PTZ treatment
Rats were injected intraperitoneally with PTZ (40 mg/kg body
Results
In PTZ-treated and control rats, mean densities (fmol/mg protein) of AMPA, kainate, NMDA, A1, GABAA, BZ and GABAB binding sites were measured in the amygdala, the piriform, entorhinal, somatosensory and retrosplenial granular cortices as well as in the hippocampal CA1 region (CA1) and the dentate gyrus (Fig. 1, Fig. 2, Table 2). Significant differences between control and PTZ-treated rats were found for kainate, NMDA, BZ and A1 binding sites (Fig. 3).
High densities of AMPA receptors were
Discussion
The aim of this study was to determine the effects of repeated PTZ-induced seizures on the densities of binding sites of different excitatory and inhibitory neurotransmitter receptors in the adult rat brain. A reduction of kainate receptor binding sites, but an increase of NMDA receptors as well as of BZ binding sites was found in most brain regions studied. The density of A1 receptor binding sites was reduced in a few regions.
PTZ was introduced as a GABAA antagonist (Macdonald and Barker, 1978
Acknowledgments
The authors appreciate the technical support by M. Cremer, S. Krause and S. Wilms. We would also like to thank A. Bauer for helpful comments on adenosine receptor properties.
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