Neuron
Volume 85, Issue 2, 21 January 2015, Pages 275-288
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DeCoN: Genome-wide Analysis of In Vivo Transcriptional Dynamics during Pyramidal Neuron Fate Selection in Neocortex

https://doi.org/10.1016/j.neuron.2014.12.024Get rights and content
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Highlights

  • Purification of neuronal subtypes without genetic labeling

  • Transcriptome dynamics of neocortical projection neurons during corticogenesis

  • Identify lncRNAs with restricted temporal and neuron subtype specific expression

  • Transcriptome data presented in an interactive online resource: DeCoN (http://decon.rc.fas.harvard.edu/)

Summary

Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long noncoding RNAs with restricted temporal and cell-type-specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multifaceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.

Cited by (0)

5

Co-first author

6

Present address: Pittsburgh Institute for Neurodegenerative Diseases, Departments of Neurology and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA

7

Present address: Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA