Dactylosporina brunneomarginata sp. nov., belonging to the family Physalacriaceae (Agaricales) is described and illustrated. This fungus differs from previously described species of Dactylosporina macroscopically by its viscid pileus and stipe surface when wet, the lamellae with dark brown edges, and microscopically by its gloeocystidioid pileo-, cheilo-, and caulocystidia with olive to yellow-brown oily content. This is the first report on Dactylosporina from Asia.