Elsevier

Materials Science and Engineering: C

Volume 82, 1 January 2018, Pages 354-362
Materials Science and Engineering: C

Preparation and evaluation of squid ink polysaccharide-chitosan as a wound-healing sponge

https://doi.org/10.1016/j.msec.2017.08.068Get rights and content

Highlights

  • The squid ink polysaccharide-chitosan sponge (SIP-CS) is obtained by CCD.

  • SIP-CS could adsorb hemocytes and stop bleeding rapidly.

  • IP-CS could promote reepithelization and repair of the epidermis and dermis.

Abstract

A new type of wound healing agent was developed using two marine biomaterials (squid ink polysaccharide and chitosan) as carriers and calcium chloride as an initiator for coagulation. Based on central composite design-response surface methodology, comprehensive evaluation of appearance quality for composite sponges and water absorbency were used as evaluation indices to identify the optimized preparation conditions and further evaluate the performance of the squid ink polysaccharide-chitosan sponge (SIP-CS). The optimized formulation of SIP-CS was as follows: chitosan concentration, 2.29%; squid ink polysaccharide concentration, 0.55%; and calcium chloride concentration, 2.82%, at a volume ratio of 15:5:2. SIP-CS was conducive to sticking on the wound, characterized by the spongy property, strong absorptivity, and tackiness. Rabbit ear arterial, hepatic, and femoral artery hemorrhage experiments indicated that, compared with chitosan dressings and absorbable gelatin, the hemostatic times were shorter and the bleeding volume was smaller. Furthermore, SIP-CS absorbed a large amount of hemocytes, leading to rapid hemostasis. The healing areas and wound pathological sections in scalded New Zealand rabbits indicated that SIP-CS promoted wound healing more rapidly than chitosan and better than commercially available burn cream. Thus, SIP-CS is a good wound healing agent for rapid hemostasis, promoting burn/scalded skin healing, and protecting from wound infection.

Keywords

Squid ink polysaccharide
Chitosan
Central composite design-response surface methodology
Hemostasis
Scalds
Skin healing

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