Microcontact imprinted surface plasmon resonance sensor for myoglobin detection

https://doi.org/10.1016/j.msec.2013.04.041Get rights and content

Highlights

  • Micro-contact imprinted surface plasmon resonance sensor.

  • Real-time myoglobin detection in the serum taken from a patient with acute myocardial infarction

  • Reproducible results for consecutive myoglobin solution supplement

  • LOD and LOQ values of the SPR sensor were determined to be 26.3 and 87.6 ng/mL.

  • The SPR sensor has potential for myoglobin sensing during acute MI cases.

Abstract

In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH 7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.

Keywords

Molecularly imprinted polymer
Microcontact imprinting
SPR sensor
Myoglobin
Nanofilm

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