Co-carrying of KPC-2, NDM-5, CTX-M-3 and CTX-M-65 in three plasmids with serotype O89: H10 Escherichia coli strain belonging to the ST2 clone in China
Introduction
It is noteworthy that co-carrying of a large number of antibiotics resistance genes by a single pathogenic bacterial isolate has become a major threat to public health [1,2]. Particularly, coproduction of multiple carbapenemases genes (blaNDM and blaKPC) brings a significant challenge for clinical treatment [[3], [4], [5]]. Even more worryingly, most of those resistance genes are found to be carried by a variety of mobile genetic elements (transposons, integrons, integrative and conjugative elements, genomic islands and plasmids), which will profoundly facilitate the inter- and intra-species dissemination of resistance [6,7].
Escherichia coli, a kind of enterobacteriaceae, common opportunistic pathogen normally causes a wide range of infection diseases such as urinary tract infections and other community-acquired infections [[8], [9], [10], [11]]. Especially, the carbapenemases producing E. coli pose a serious threat to public health. Strikingly, the emergence of carbapenem-resistant enterobacteriaceae has been identified as an urgent threat to human health according to the US Centers for Disease Control and Prevention [12]. In addition to the carbapenem-resistant enterobacteriaceae, the carbapenem resistance genes (blaKPCs, blaNDMs, blaIMPs blaVIMs and blaOXA48 like) and other genes (resistance to cephalosporins, chloramphenicol, chromate, rifampicin and sulphonamides) can also be carried by other bacterial species and making them highly resistant to almost all kinds of antibiotics [[13], [14], [15], [16]]. The plasmids carrying the blaKPC-2 or blaNDM gene have been widely detected in enterobacteriaceae species. In this study, we present the genetic characteristics of one E. coli strain ZSH6 (serotype O89: H10) which harbors three different plasmids co-carrying a large number of resistance genes including blaKPC-2, blaNDM and blaCTX-M.
Section snippets
Bacterial strain, antimicrobial susceptibility testing, resistance genes detection
Escherichia coli isolate ZSH06 was obtained from a blood sample of a 82-year-old man,a long-stay patient, admitted to the intensive care unit of Henan People's Hospital (Henan, China) pulmonary tumor with severe pulmonary infection in April 2018. Routine species identification and antimicrobial susceptibility testing were carried out by the Vitek2 system (BioMérieux, France). The minimal inhibitory concentrations (MICs) of ten antimicrobial agents including (imipenem, meropenem, cefepime,
Results and discussion
E. coli strain ZSH6 isolated from the blood sample showed high resistance to all the tested antimicrobial agents (imipenem, meropenem, cefepime, ceftazidime, cefotaxime, ceftriaxone, piperacillin-tazobactam, ampicillin, gentamicin, amikacin and aztreonam) in this study (Table .2). By using resistance genes detection, E. coli strain ZSH6 co-carried the blaKPC-2, blaNDM-5 and blaCTX-M resistance gene. By using the conjugation experiments, three different kinds of transconjugants, one carried the
Conclusions
We identified a ST2, serotype O89: H10 carbapenem-resistant Escherichia coli co-carrying of the blaKPC-2, blaNDM-5 and other resistance genes including blaCTX-M-65, blaCTX-M-3 in three different plasmid. This type of isolates may pose huge threat to public health and is a serious challenge for the resistance control and treating the infections caused by these bacteria.
Conflicts of interest
The authors declare that they have no competing interests.
Nucleotide sequence accession number
This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession QVOP00000000.
Funding
This research was funded by the National Natural Science Foundationof China (31500114, 31700111) and by a grant from the Sichuan Province Science and Technology project (2016JY0223), and Southwest Medical University Natural Science Foundation [2018LZXNYD-ZK51] and Health and Family Planning Commission of Sichuan Province grant (16PJ538).
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Li Fu, Shanmei Wang and Zhikun Zhang and contributed equally to this work.