Method ArticleRppH can faithfully replace TAP to allow cloning of 5′-triphosphate carrying small RNAs
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Graphical abstract
Method name
RppH treatment of small RNAs prior to sequencing library preparation
Keywords
RNA 5′ pyrophosphohydrolase
RppH
Tobacco acid pyrophosphatase
TAP
Small RNAs
C. elegans
Sequencing
22G RNA
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These authors contributed equally to this work.
© 2019 The Authors. Published by Elsevier B.V.