Elsevier

Metabolism

Volume 53, Issue 9, September 2004, Pages 1118-1120
Metabolism

F2 isoprostane is already increased at the onset of type 1 diabetes mellitus: Effect of glycemic control

https://doi.org/10.1016/j.metabol.2004.04.005Get rights and content

Abstract

Much evidence has suggested that oxidative stress (OS) may play a role in the pathogenesis of diabetic complications. However, the relationship between hyperglycemia and OS is inconsistent in diabetic clinical studies. The aim of this study was to evaluate the effect of normalization of blood glucose levels on urinary 8-epi-prostaglandin F (8-epi-PGF) excretion at the onset of type 1 diabetes. We studied 14 type 1 diabetic patients (50% males; mean age, 24.3 ± 4.9 years) and 14 control subjects matched by age and body mass index. A 24-hour urine collection was performed to determine 8-epi-PGF as an integrated index of OS production at baseline, before starting insulin therapy, and 16 weeks later. Insulin treatment induced a significant reduction in glycosylated hemoglobin (HbA1c) (from 11.5% to 5.4% P = .0001), triglycerides (from 1.0 to 0.8 mmol/L, P = .002), and an increase in high-density lipoprotein (HDL)-cholesterol levels (from 1.1 to 1.5 nmol/L, P = .01) at week 16. This improvement in metabolic control was associated with a statistically significant reduction in 8-epi-PGF values (from 92.0 ± 41.5 to 66.9 ± 28.9 pg/mg urinary reatinine excretion, P = .015), although compared with the control group, 8-epi-PGF values remained higher in diabetic patients (66.9 ± 28.9 v 39.1 ± 13.8 pg/mg creatinine, P = .004). Enhanced OS is present in early clinical phases of type 1 diabetes, and the amelioration in metabolic control is associated with improvement in this pathogenic pathway.

Section snippets

Materials and methods

We studied 14 type 1 diabetic patients recruited from an adult Diabetes Unit at the onset of the disease (50% males; mean age, 24.3 ± 4.9 years) and 14 healthy volunteers (42% males; mean age, 27.4 ± 3.5). The 2 groups were matched with respect to age and body mass index. All patients were nonsmokers and were normotensive (<130/80 mm Hg). Diagnosis of type 1 diabetes was achieved according to the National Diabetes Data Group criteria.7 Patients were excluded if, at admission, they presented

Results

Fourteen patients who fulfilled inclusion criteria were evaluated. At admission all patients presented hyperglycemia and 10 ketonuria. All patients showed autoantibodies related to type 1 diabetes, 83% of which were anti-GAD+ and 66% were IA2+. No differences were noted in any clinical variable evaluated between type 1 diabetic patients and control subjects. In relation to control subjects, urinary 8-epi-PGF excretion was significantly higher in type 1 diabetics (66.9 ± 28.9 v 39.1 ± 13.8

Discussion

At present, numerous data support the concept that LP, a marker of OS, is enhanced in diabetes mellitus.1, 2, 3, 4, 5 However, some studies have shown controversial results, probably related to shortcomings in the methods used, because most studies use indirect measurements of LP including susceptibility of LDL to in vitro oxidation or malondialdehyde determination, techniques which are not very specific or accurate.8 F2-isoprostanes are produced in vivo by cycloxygenase-independent free

References (19)

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