Fig. 1. Influence of different concentrations of LPS (25, 50, 75 and 100 μg/ml) on ATP (A), ADP (B) and AMP (C) hydrolyses in lymphocytes from mesenteric lymph nodes of rats. The control of enzymatic activities in lymphocytes was 0.38 ± 0.05, 0.25 ± 0.04 and 0.13 ± 0.012 nmol Pi min^− 1 · 10^− 6 cells for ATP, ADP and AMP, respectively. The data represent a mean ± SD (n = 5 at least). Statistical analyses were performed by one-way analysis of variance (ANOVA), followed by a Duncan multiple range test, considering P < 0.05 as significant ().

Fig. 2. Influence of different concentrations of LPS (25, 50, 75 and 100 μg/ml) on ρ-Nph-5′-TMP hydrolysis from blood serum fraction of rats. The control of specific activity in serum was 3.3 ± 0.12 nmol ρ-nitrophenol min^− 1 mg^− 1 of protein. The data represent a mean ± SD (n = 5 at least). Statistical analyses were performed by one-way analysis of variance (ANOVA), followed by a Duncan multiple range test, considering P < 0.05 as significant ().

Fig. 3. ATP (A), ADP (B) and AMP (C) hydrolyses in lymphocytes from mesenteric lymph nodes of rats after 24 and 48 h of endotoxemia induction. The control of enzymatic activities in lymphocytes was 0.42 ± 0.12, 0.29 ± 0.06 and 0.14 ± 0.013 nmol Pi min^− 1 · 10^− 6 cells for ATP, ADP and AMP, respectively. The data represent a mean ± SD (n = 5 at least). Statistical analyses were performed by one-way analysis of variance (ANOVA), followed by a Duncan multiple range test, considering P < 0.05 as significant ().

Fig. 4. ATP (A), ADP (B) and AMP (C) hydrolyses from blood serum fraction of rats after 24 and 48 h of endotoxemia induction. The control of specific activities in serum was 1.05 ± 0.23, 1.11 ± 0.23, 1.16 ± 0.26 nmol Pi min^− 1 mg^− 1 of protein for ATP, ADP and AMP, respectively. The data represent a mean ± SD (n = 5 at least). Statistical analyses were performed by one-way analysis of variance (ANOVA), followed by a Duncan multiple range test, considering P < 0.05 as significant ().

Fig. 5. ρ-Nph-5′-TMP hydrolysis from blood serum fraction of rats after 24 and 48 h of endotoxemia induction. The control of specific activity in serum was 2.99 ± 0.3 nmol ρ-nitrophenol min^− 1 mg^− 1 of protein. The data represent a mean ± SD (n = 5 at least). Statistical analyses were performed by one-way analysis of variance (ANOVA), followed by a Duncan multiple range test, considering P < 0.05 as significant ().

Fig. 6. Gene expression patterns after LPS treatment for NTPDase1 (A and B), NTPDase2 (C and D), NTPDase3 (E and F), 5′-nucleotidase (G and H) and β-actin in mesenteric lymph nodes of rats. Rats were injected with LPS and after 24 and 48 h, the mesenteric lymph nodes were excised and total RNA was isolated being subjected to RT-PCR for the indicated targets. Three independent experiments were performed, with entirely consistent results.