Abstract

This article describes the performance of the two protease sequencing modules available in the Trugene HIV-1 genotyping Kit on a sample population with a high prevalence of HIV-1 non-B subtypes (n = 110). The relevance of the algorithm recommended by the kit was also evaluated. The results indicated a high sequencing failure rate of the PR module (34%). Forty-five percent of the failed sequences derived from non-B subtype viruses. Furthermore, no PR sequence could be obtained from any of the HIV-1 subtype A and C infected samples that were tested. In contrast, a sequence could be obtained from the entire panel using the P2 module. The data indicated that the high rate of sequencing failures of the PR module was related to both the HIV-1 non-B subtypes as well as lower levels of RNA viral load. In six out of the 73 samples for which both protease modules were successful, discrepancies between the two protease sequences were observed, which led to discordant resistance reports in two cases. The data highlight the problems and the clinical implications that may occur during resistance genotyping of clinical samples with a high prevalence of HIV-1 non-B subtypes.

Keywords: Trugene; Genotyping; HIV-1; Subtype; Protease

Article Outline

1. Introduction

2. Materials and methods

2.1. Patients and samples

2.2. Laboratory tests

2.2.1. HIV-1 RNA viral load

2.2.2. Resistance genotype

2.2.3. HIV-1 genetic subtypes

2.3. Statistical analysis

3. Results

3.1. RNA viral load and HIV-1 subtypes

3.2. Resistance genotyping

3.3. Resistance genotype as a function of HIV-1 subtype and HIV-1 RNA viral load

4. Discussion

Acknowledgements

References