Elsevier

Journal of Proteomics

Volume 72, Issue 1, 15 February 2009, Pages 71-81
Journal of Proteomics

Review
Unravelling the nuclear matrix proteome

https://doi.org/10.1016/j.jprot.2008.09.005Get rights and content

Abstract

The nuclear matrix (NM) model posits the presence of a protein/RNA scaffold that spans the mammalian nucleus. The NM proteins are involved in basic nuclear function and are a promising source of protein biomarkers for cancer. Importantly, the NM proteome is operationally defined as the proteins from cells and tissue that are extracted following a specific biochemical protocol; in brief, the soluble proteins and lipids, cytoskeleton, and chromatin elements are removed in a sequential fashion, leaving behind the proteins that compose the NM. So far, the NM has not been sufficiently verified as a biological entity and only preliminary at the molecular level. Here, we argue for a combined effort of proteomics, immunodetection and microscopy to unravel the composition and structure of the NM.

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Acknowledgements

We would like to thank Jeffrey A. Nickerson for generously providing us with the picture of the nuclear matrix structure shown in Fig. 1. We would like to thank Marc Warmoes for performing overlap analysis of NM parts lists.

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