PathologyThe CCL2-CCR2 Axis in Lymph Node Metastasis From Oral Squamous Cell Carcinoma: An Immunohistochemical Study
Section snippets
Patients and Tissue Specimens
Forty-one cases of OSCC were obtained from the files of the Division of Oral Pathology and Bone Metabolism at the Nagasaki University Graduate School of Biomedical Sciences (Nagasaki, Japan) for histologic and immunohistochemical examination. These included 28 cases of OSCC resection with simultaneous neck dissection and 13 cases of radical neck dissection after resection of a primary tumor. Of the 28 cases of OSCC with simultaneous neck dissection, 13 exhibited no lymphatic metastasis and 15
Results
The HE-stained sections were examined to confirm the presence of sinus histiocytosis in the regional lymph nodes of patients with OSCC. The tissue sections frequently exhibited sinus histiocytosis; in particular, marginal sinus histiocytosis was detected in 93.3% of metastatic cases with concurrent resection of the primary tumor and 84.6% of postresection metastatic cases (Fig 1A). In contrast, 23.1% of metastasis-free cases exhibited sinus histiocytosis, which was markedly lower than in the
Discussion
Metastasis of tumor cells to regional lymph nodes is an important prognostic factor in determining the stage of various malignancies, including OSCC. For the establishment of lymphatic metastasis, interactions between cancer cells and various immune cells by the signaling of cytokines and chemokines are required.21, 22 These immune cells and mesenchymal cells (eg, CAFs) prepare the microenvironment to cultivate cancer stem cells and further support tumor development.16, 17, 23 A particular
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This work was supported by the JSPS KAKENHI (grant JP25462864).
Conflict of Interest Disclosures: None of the authors have any relevant financial relationship(s) with a commercial interest.