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doi:10.1016/j.jneumeth.2004.05.008 
Copyright © 2004 Elsevier B.V. All rights reserved.
Dynamic susceptibility contrast-enhanced perfusion MR imaging at 1.5 T predicts final infarct size in a rat stroke model
Received 23 February 2004;
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Abstract
The purpose of the present animal experiment was to determine whether source images from dynamic susceptibility contrast-enhanced perfusion weighted imaging (DSC-PWI) at a 1.5 T MR scanner, performed early after photochemically induced thrombosis (PIT) of cerebral middle artery (MCA), is feasible to predict final cerebral infarct size in a rat stroke model. Fifteen rats were subjected to PIT of proximal MCA. T2 weighted imaging (T2WI), diffusion-weighted imaging (DWI), and contrast-enhanced PWI were obtained at 1 h and 24 h after MCA occlusion. The relative lesion size (RLS) was defined as lesion volume/brain volume × 100% and measured for MR images, and compared with the final RLS on the gold standard triphenyl tetrazolium chloride (TTC) staining at 24 h. One hour after MCA occlusion, the RLS with DSC-PWI was 24.9 ± 6.3%, which was significantly larger than 17.6 ± 4.8% with DWI (P < 0.01). At 24 h, the final RLS on TTC was 24.3 ± 4.8%, which was comparable to 25.1 ± 3.5%, 24.6 ± 3.6% and 27.9 ± 6.8% with T2WI, DWI and DSC-PWI respectively (P > 0.05). The fact that at 1 h after MCA occlusion only the displayed perfusion deficit was similar to the final infarct size on TTC (P > 0.05) suggests that early source images from DSC-PWI at 1.5 T MR scanner is feasible to noninvasively predict the final infarct size in rat models of stroke.
Author Keywords: Ischemia; Stroke; Models; Magnetic resonance imaging; Contrast enhancement; Experimental
Article Outline
- 1. Introduction
- 2. Materials and methods
- 2.1. Rat PIT stroke model with proximal MCA obstruction
- 2.2. MR imaging techniques
- 2.3. Histochemical staining
- 2.4. Image analysis
- 2.5. Statistical analysis
- 3. Results
- 3.1. General conditions
- 3.2. Interobserver agreement
- 3.3. MRI findings as verified with histochemical staining
- 3.4. One hour after MCA occlusion
- 3.5. Twenty-four hours after MCA occlusion
- 3.6. Comparison between 1 h and 24 h
- 4. Discussion
- References
