Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats
Introduction
Iodine is a trace element which is essential for health and the development of humans and animals, particularly mammals (Dong et al, 2009, WHO, 2014). This trace element is necessary for the biosynthesis of thyroid hormones: thyroxin (T4) and triiodothyronine (T3). Iodine deficiency contributes to a wide spectrum of diseases: from endemic goiter to impaired memory and cognitive function/mental disorder (Dong et al, 2009, Melse-Boonstra, Jaiswal, 2010). Developing fetuses are most susceptible to the lack of iodine which leads to numerous neurological disorders beginning with lowered intelligence quotient scores to severe mental retardation (cretinism). Children are particularly liable to iodine deficiency in every stage of development (Melse-Boonstra, Jaiswal, 2010, Skeaff, 2011, Walker et al, 2007).
According to the current World Health Organization (WHO) data, the deficiency of iodine was ascertained in 1993 in 110 countries around the World. In 2013 this number decreased to 31 countries. Deficiency of iodine is still common in Asia, Africa and Eastern Europe. It has been estimated that about 1.88 billion people suffer from it (WHO/UNICEF, 2007, WHO, 2014). Fortification of salt with iodine has been a major reason for the reduction of iodine deficiency in many countries in Europe (i.e. Austria, Finland, Poland, Switzerland, Norway), Asia (i.e. China, Indonesia, India), and Latin America (i.e. Ecuador, Peru, Venezuela) mainly due to relatively high levels of salt intake in daily diets (7.9–16.0 g NaCl/day), (Andersson et al, 2010, WHO, 2008, WHO, 2014). However, this level of salt consumption exceeds the amount recommended by WHO (5 g NaCl/day). Excessive salt intake is one of the major causes of cardiovascular diseases (i.e. hypertension, stroke, atherosclerosis) and other diseases such as stomach cancer (WHO, 2008, Maillot, Drewnowski, 2012). Global economic and social effects of these disorders are enormous. For that reason WHO established the “2008–2013 Action Plan for the Global Strategy for the Prevention and Control of Noncommunicable Diseases”. This particular programme included guidelines for reducing table salt consumption as well as the development/identification of alternative ways of iodine reduction in the human daily diet (WHO, 2008, WHO, 2014). One of these methods (apart from iodized mineral water, milk, flour, bread etc.) can be plant fortification-biofortification with iodine trace element during plant cultivation (Blasco et al, 2008, Caffagni et al, 2011, Smoleń et al, 2011, Strzetelski et al, 2010, Ujowundu et al, 2010, Voogt et al, 2010).
The objective of this study was to evaluate the effect of the addition of lettuce biofortified with iodine in KI form, on iodine content in selected tissues, lipid profile, thyroid hormone concentration and mRNA expression of selected genes involved in iodine metabolism in Wistar rats.
Section snippets
Plant material
Lettuce ‘Melodion’ cv. was cultivated in the spring season of 2012 in a field experiment on heavy soil (24% sand, 23% dust and 53% loam) characterized by: pH(H2O) 6.02, pH(KCl) 4.97, EC (electrical conductivity) 0.10 mS cm−1 and the content of: organic matter −2.33%, N as a ammonium nitrate (NH4NO3; NO3− and NH4+ in proportion 1:1) −13.4 mg, P-32.7 mg, K-168.6 mg, Mg-194.2 mg, Ca-2089.3 mg and S-41.4 mg in 1 dm3 of soil. One day before plant seedling into the field, mineral fertilizers:
Results
Biofortification of lettuce with iodine did not affect basic chemical composition when compared to the control lettuce (Table 2). Iodine content significantly increased in the biofortified lettuce when compared to the control lettuce.
The body weight gain, feed efficiency ratio (FER), heart and kidney weights were not affected by various dietary treatments. Liver weight was significantly higher (P ≤ 0.05) in the rats fed the control diet when compared to the liver of the rats fed the BFL diet as
Discussion
Basic chemical composition of the biofortified lettuce was not affected by soil fertilization with iodine. The biofortified lettuce had a higher content of iodine in comparison to the control lettuce (Table 2). According to the current dietary recommendations we should consume 5 portions of vegetables and fruits per day (WHO/FAO, 2003). Our results shows that a 100 g of dried, biofortified lettuce will deliver about 500 µg of iodine, and only 30 g will deliver the Recommended Nutrient Intake
Acknowledgement
This work was financed by the 2012–2015 Polish National Science Center – grant no. DEC-2011/03/D/NZ9/05560 “I and Se biofortification of selected vegetables, including the influence of these microelements on yield quality as well as evaluation of iodine absorption and selected biochemical parameters in rats fed with vegetables biofortified with iodine”.
References (38)
- et al.
Epidemiology of iodine deficiency: Salt iodisation and iodine status
Best Practice & Research Clinical Endocrinology & Metabolism
(2010) - et al.
Effects of dietary fibers on disturbances clustered in the metabolic syndrome
The Journal of Nutritional Biochemistry
(2008) - et al.
Effect of long term administration of resveratrol on lipid concentration in selected organs and liver's histology in rats fed high fructose diet
Journal of Functional Foods
(2013) - et al.
Thyroid hormone receptor isoform-specific modification by small ubiquitin-like modifier (SUMO) modulates thyroid hormone-dependent gene regulation
Journal of Biological Chemistry
(2012) - et al.
A conflict between nutritionally adequate diets and meeting the 2010 dietary guidelines for sodium
American Journal of Preventive Medicine
(2012) - et al.
Iodine deficiency in pregnancy, infancy and childhood and its consequences for brain development
Best Practice & Research Clinical Endocrinology & Metabolism
(2010) - et al.
Child development: Risk factors for adverse outcomes in developing countries
Lancet
(2007) - et al.
Metabolic effects of dietary fiber consumption and prevention of diabetes
Journal of Nutrition
(2008) Official methods of analysis
(2006)- et al.
Deiodinases: Implications of the local control of thyroid hormone action
The Journal of Clinical Investigation
(2014)
Iodine effects of phenolic metabolism in lettuce plants under salt stress
Journal of Agricultural and Food Chemistry
Iodine biofortification and antioxidant capacity of lettuce: Potential benefits for cultivation and human health
Annals of Applied Biology
Iodine fortification plant screening process and accumulation in tomato fruits and potato tubers
Communications in Soil Science and Plant Analysis
Development of iodine deficiency resulting in hypothyroidism reduces hippocampal ERK1/2 and CREB in lactational and adolescent rats
BMC Neuroscience
Differential expression of members of the E2F family of transcription factors in rodent testes
Reproductive Biology and Endocrinology
Targeting thyroid hormone receptor-β agonists to the liver reduces cholesterol and triglycerides and improves the therapeutic index
Proceedings of the National Academy of Sciences of the United States of America
Environmental controls in IDD: A case study in Xinjiang Province of China
Podstawy analizy żywności [Basic of food analysis]
Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge
Clinical Chemistry
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