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doi:10.1016/j.jep.2006.05.009 
Copyright © 2006 Elsevier Ireland Ltd All rights reserved.
Altered expression of serum protein in ginsenoside Re-treated diabetic rats detected by SELDI-TOF MS
William C.S. Choa, 
, 
, Tai-Tung Yipb, Wai-Shing Chunga, Sally K.W. Leea, Albert W.N. Leungc, Christopher H.K. Chengd and Kevin K.M. Yuea
Received 25 November 2005;
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Abstract
Diabetes mellitus (DM) is now a global health problem, however, its pathogenesis has not yet been fully deciphered. Even though modern medicine has great contribution to the control and treatment of DM, it is still far from success to completely cure the disease. Panax ginseng C.A. Meyer (ginseng) is a well-recognized traditional Chinese medicine for treating DM in Asia. In this study, high throughput proteomic approach has been adopted to investigate the antidiabetic action of 2 weeks’ ginsenoside Re (Re, a major component of ginseng) administration to streptozotocin-induced diabetic rats. Employing surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and bioinformatics, 432 cluster peaks were detected in the samples, among them 293 potential biomarkers were found to have significant differentiations between the DM and control normal rats. When the Re-treated diabetic rats were compared to the untreated ones, a protein peak was detected to have significant alteration corresponding to Re treatment. This specific protein was found to match with C-reactive protein (CRP) in the protein database, and was subsequently validated by ELISA. This is the first study demonstrated that CRP could be altered by Re treatment, indicating that Re may improve diabetes and its complications by alleviation of inflammation.
Keywords: Diabetes mellitus; Traditional Chinese medicine; Panax ginseng; Ginsenoside Re; SELDI-TOF MS; C-reactive protein
Abbreviations: CRP, C-reactive protein; DM, diabetes mellitus; Ginseng, Panax ginseng C.A. Meyer; HPLC, high performance liquid chromatography; m/z, mass to charge; PCA, principal components analysis; Re, ginsenoside Re; ROC, receiver-operator characteristic; SELDI-TOF MS, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; STZ, streptozotocin; TCM, traditional Chinese medicine; Vehicle, polyvinylpyrrolidone-10 solution
Article Outline
- 1. Introduction
- 2. Materials and methods
- 2.1. Chemicals
- 2.2. Animal models
- 2.3. Sample preparation
- 2.4. Proteomic profiling
- 2.5. Bioinformatics
- 2.6. C-reactive protein measurement
- 2.7. Statistical analyses
- 3. Results
- 3.1. Changes in serum protein expression profile between diabetic rats and control normal rats
- 3.2. Changes in serum protein expression profile induced by treatment with ginsenoside Re
- 3.3. Protein matching and validation
- 4. Discussion
- References
