Research Letter
A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture

https://doi.org/10.1016/j.jcmgh.2018.04.008Get rights and content
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Abbreviation used in this letter

GI
gastrointestinal

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Author contributions Michael Czerwinski, Yu-Hwai Tsai, John Y. Kao, and Jason R. Spence conceived the study; Yu-Hwai Tsai, John Y. Kao, Michael Czerwinski, Angeline Wu, Lauren Marie Nowacki, Peter D. R. Higgins, Michael K. Dame, Durga Attili, Evan Hill, and Justin A. Colacino conducted the experiments; John Y. Kao and Peter D. R. Higgins obtained study permissions and collected patient samples; Yu-Hwai Tsai, Michael Czerwinski, Angeline Wu, Jason R. Spence, Lauren Marie Nowacki, Noah F. Shroyer, John Y. Kao, Peter D. R. Higgins, Michael K. Dame, Durga Attili, Evan Hill, and Justin A. Colacino analyzed the data; Michael Czerwinski and Jason R. Spence wrote the manuscript; and all authors edited the manuscript and approved the final version.

Conflicts of interest The authors disclose no conflicts.

Funding This work was supported by funds from the University of Michigan Department of Internal Medicine (J.R.S.), by the National Institute of Diabetes and Digestive and Kidney Diseases R01DK117565 (J.Y.K.), and by the University of Michigan Center for Gastrointestinal Research (National Institute of Diabetes and Digestive and Kidney Diseases 5P30DK034933).

Authors share co-first authorship.