Planar chromatography – Current practice and future prospects

https://doi.org/10.1016/j.jchromb.2022.123553Get rights and content
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Highlights

  • Methods and techniques in planar chromatography (TLC/HPTLC) are reviewed.

  • The advantages and limitations of planar chromatography are revisited.

  • New developments in bioautography for “effect testing” are highlighted.

  • Advances in the detection/identification analytes by TLC/MS are described.

  • Future directions in the development and use of planar chromatography are considered.

Abstract

Planar chromatography, in the form of thin-layer or high-performance thin-layer chromatography (TLC, HPTLC), continues to provide a robust and widely used separation technique. It is unrivaled as a simple and rapid qualitative method for mixture analysis, or for finding bioactive components in mixtures. The format of TLC/HPTLC also provides a unique method for preserving the separation, enabling further investigation of components of interest (including quantification/structure determination) separated in both time and space from the original analysis. The current practice of planar chromatography and areas of development of the technology are reviewed and promising future directions in the use of TLC/HPTLC are outlined.

Keywords

TLC
HPTLC
TLC/MS
Automation
Effect-directed detection
Imaging

Data availability

No data was used for the research described in the article.

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