Development of a highly efficient indigo dyeing method using indican with an immobilized β-glucosidase from Aspergillus niger
Section snippets
Materials
Three commercially available β-glucosidases from Almond, Tricoderma viride, and A. niger were used for the hydrolysis of indican. Partially purified Almond β-glucosidase and T. viride β-glucosidase were obtained from Wako Pure Chemical Industries (Osaka, Japan) while the crude A. niger enzyme preparation (Novozym 188) was obtained from Sigma-Aldrich (St. Louis, MO). Indican and indigo were obtained from Sigma-Aldrich and Wako, respectively. Toyopearl DEAE 650 S and Bio-Gel P-100 were from Tosoh
Selection of β-glucosidase
Three types of commercially available β-glucosidases, i. e., Almond, T. viride, and A. niger β-glucosidases were examined without further purification in terms of activity and thermal stability, particularly, in an acidic pH region. The specific activities of the Almond, T. viride, and A. niger β-glucosidases measured at pH 3 and at 30 °C were approximately 20, 0.8, and 1.1 U/mg-protein, respectively. The Almond enzyme showed the highest specific activity among the three enzyme preparations at
Acknowledgments
This work was financially supported in part by the Program for Regional Resource Utilization R&D Program (2007–2008, Ministry of Economy, Trade and Industry).
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Green production of indigo and indirubin by an engineered Baeyer–Villiger monooxygenase
2022, Biocatalysis and Agricultural BiotechnologyCitation Excerpt :Indigo is a blue-colored molecule that gives the name to this class of dyes and is one of the oldest and most widely used dyes by mankind (Gaboriaud-Kolar et al., 2014). Indigo blue has been historically extracted from Indigofera tinctoria that can be exploited for the production of indigo due to the presence of indican (indolyl-β-d-glucopyranoside), an l-tryptophan derivate and indigotin (chemical responsible for blue color of indigo) through saccharification and oxidation reactions (Warzecha et al., 2007; Song et al., 2010; K. Choi, 2020). In 1897 BASF established an industrial process to produce indigo from aniline, which is still to date the basis for the process used for the production of indigo (K. Choi, 2020).
A review of recent progress in the synthesis of bio-indigoids and their biologically assisted end-use applications
2020, Dyes and PigmentsCitation Excerpt :Natural indigo has historically been extracted from the leaves of plants such as Indigofera tinctoria, with the inclusion of more species as time has elapsed [2,3]. The synthesis of indigo in plants begins with indican (indolyl-β-d-glucopyranoside), an L-tryptophan derivative, and blue-colored indigotin is synthesized via oxidation of indoxyl (3-hydroxyindole), generated via a saccharification reaction [4,5]. With the advancement of chemical synthetic capabilities and the increasing demand for indigo dye, the development of synthetic technologies for the production of indigo from various petrochemical-based raw materials began to replace natural extraction methods.
Temperature sensitivity of mineral-enzyme interactions on the hydrolysis of cellobiose and indican by β-glucosidase
2019, Science of the Total EnvironmentCitation Excerpt :Indeed, previous studies have examined the substrate affinity toward β-glucosidase by comparing various substrate compounds, including cellobiose, indican, and pNPG, in non-mineral solutions (Dekker, 1986; Kempton and Withers, 1992; Chauve et al., 2010; Nascimento et al., 2010). Despite different experimental conditions, the reported kinetic parameters including Michaelis-Menten constant (Km) and maximum velocity (Vm) were generally larger for cellobiose than for indican and pNPG (Dekker, 1986; Maugard et al., 2002; Chauve et al., 2010; Song et al., 2010), consistent with the kinetics patterns observed in the present study (Figs. 2 and 3). Hydrolysis of cellobiose and indican by β-glucosidase breaks the glycosidic bond.
Improving the performance of immobilized β-glucosidase using a microreactor
2018, Journal of Bioscience and BioengineeringDiversity, Application, and Synthetic Biology of Industrially Important Aspergillus Fungi
2017, Advances in Applied Microbiologyβ-Glucosidase from Aspergillus
2016, New and Future Developments in Microbial Biotechnology and Bioengineering: Aspergillus System Properties and Applications