Prime-O-glucosylcimifugin attenuates lipopolysaccharide-induced acute lung injury in mice

https://doi.org/10.1016/j.intimp.2013.04.014Get rights and content

Highlights

  • Prime-O-glucosylcimifugin regulated the expression of TNF-α, IL-1β, IL-6, and IL-10.

  • Prime-O-glucosylcimifugin inhibited the LPS-induced MAPK and NF-κB activation.

  • Prime-O-glucosylcimifugin protects mice from LPS-induced acute lung injury.

Abstract

Prime-O-glucosylcimifugin is an active chromone isolated from Saposhnikovia root which has been reported to have various activities, such as anti-convulsant, anticancer, anti-inflammatory properties. The purpose of this study was to evaluate the effect of prime-O-glucosylcimifugin on acute lung injury (ALI) induced by lipopolysaccharide in mice. BALB/c mice received intraperitoneal injection of Prime-O-glucosylcimifugin 1 h before intranasal instillation (i.n.) of lipopolysaccharide (LPS). Concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and interleukin (IL)-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Pulmonary histological changes were evaluated by hematoxylin–eosin, myeloperoxidase (MPO) activity in the lung tissue and lung wet/dry weight ratios were observed. Furthermore, the mitogen-activated protein kinases (MAPK) signaling pathway activation and the phosphorylation of IκBα protein were determined by Western blot analysis. Prime-O-glucosylcimifugin showed promising anti-inflammatory effect by inhibiting the activation of MAPK and NF-κB signaling pathway.

Keywords

Prime-O-glucosylcimifugin
Lipopolysaccharide
Acute lung injury

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1

These authors contributed equally to this work.

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