International Journal of Oral and Maxillofacial Surgery
Research PaperClinical PathologyBacterial profile and bone healing in rats receiving cancer therapeutic doses of bisphosphonates and corticosteroids: a pilot study
Section snippets
Rats with BRONJ-like disease
The Animal Use Protocol was approved by the necessary authorities. A total of 14 retired breeder female Sprague-Dawley rats (4–6 months old) were divided randomly into control (n = 6) and experimental (n = 8) groups. Control rats received no medication and experimental rats received zoledronic acid (ZA; 125 μg/kg twice a week) and dexamethasone (DX; 5 mg/kg once a week) for a total of 12 weeks. In a previous work by the present investigators, rats exposed to this drug combination consistently
Microbiological results
Of the 43 different microbial species investigated, a total of 39 were detected on the exposed bone and 26 on the adjacent teeth of the experimental rats treated with ZA+DX, and 40 were detected on the teeth of control rats that received no treatment. Staphylococcus pasteuri, Streptococcus parasanguinis, Streptococcus mitis, Streptococcus gordonii, and Streptococcus oralis species had the highest mean proportions. S. pasteuri, S. parasanguinis, and S. mitis were detected on all exposed bone in
Discussion
The combined administration of ZA+DX associated with trauma to the jaw bone, caused by tooth extraction, resulted in exposed bone resembling BRONJ in all experimental rats. A DNA checkerboard technique using 43 genomic DNA probes prepared from human oral bacteria and fungi relevant to human health was employed to detect changes in the bacterial species colonizing the teeth of the ZA+DX rats. A similarity in bacterial profile of the exposed bone and teeth was also noted, and no loss of alveolar
Funding
Zaher Jabbour has received awards from the Fonds de Recherche en Santé du Québec (FRSQ), the FRSQ-Réseau de Recherche en Santé Buccodentaire et Osseuse (RSBO), and the Fondation de l’Ordre des Dentistes du Québec (FODQ). The study was made possible through operating funds from FODQ and Synthes Canada, and infrastructure support from the FRQ-S-RSBO and the Jo Miller Orthopaedic Research Fund.
Competing interests
None.
Ethical approval
The Animal Use Protocol was approved by the University Animal Care Committee to perform the current experiment according to the Principles of Laboratory Animal Care established by the Canadian Council on Animal Care (CCAC).
Patient consent
Not required.
Acknowledgements
The technical assistance provided by Ailian Li for the histological staining was greatly appreciated.
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