International Journal of Oral and Maxillofacial Surgery
Research PaperWound HealingEffects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid
Section snippets
Materials and methods
This research was developed in the university laboratory. All experiments were performed using a human gingival fibroblast continuous cell line (HGF). Cells were exposed to ZA and irradiated with a laser diode prototype according to selected parameters. The experimental and control groups are described in Table 1.
Results
For ZA-treated cells, we observed a significant decrease in cell proliferation compared with the control group (P < 0.05). The ZA-treated gingival fibroblasts that were subjected to LLLT presented proliferation rates similar to those of ZA-treated cells, at both energy doses tested (P > 0.05).
When the cultured cells were subjected solely to LLLT, cell proliferation was increased at both energy doses (P < 0.05). The highest cell proliferation rate was observed at 3 J/cm2 (Table 3).
Concerning cell
Discussion
Low-level laser irradiation has been used as an adjuvant therapy for bisphosphonate-induced osteonecrosis, mainly due to its positive effects against pain, its capacity to reduce oedema formation, and a notable stimulation of tissue healing.9, 10, 11, 12
The present study demonstrated that ZA significantly decreased the proliferation of cultured gingival fibroblasts and increased cell apoptosis. Similar data have been reported in previous studies,8, 19, 21 in which the authors have stated that
Funding
The authors acknowledge the Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (grants 2009/54722-1 and BP.IC: 2010/08933-8) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (grant 301029/2010-1) for financial support.
Competing interests
The authors declare no conflict of interest.
Ethical approval
Ethical approval for fibroblast isolation was obtained from the Piraciaba Dental School Ethics Committee (UNICAMP: 64/99).
Patient consent
Not required.
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