Expression of five frizzleds during zebrafish craniofacial development
Section snippets
Results and discussion
Although initially discovered in Drosophila (Gubb and Garcia-Bellido, 1982), the Wnt/Planar Cell Polarity (PCP) pathway has since been proven to be critical in establishing and maintaining epithelial and mesenchymal cell polarity in multiple organisms. In the zebrafish, Danio rerio, the PCP pathway regulates convergence and extension movements and organ development (Solnica-Krezel et al., 1996, Rauch et al., 1997, Heisenberg et al., 2000, Topczewski et al., 2001, Dale et al., 2009). Several
Zebrafish maintenance, embryo production and staging
AB (ZDB-GENO-960809-7), wnt5bta89 (ZDB-GENE-980526-87), gpc4m818 (ZDB-GENE-011119-1), wnt11tz216 (ZDB-GENE-990603-12) and Tg(fli1a:EGFP)y1/+ (ZDB-GENO-070209-102) (Lawson and Weinstein, 2002) zebrafish lines were maintained as described in Topczewski et al. (2001). Embryos were collected from natural spawning and staged according to morphology described in Kimmel et al. (1995).
Nomenclature
fzd7a was originally referred to as fz7 (El-Messaoudi and Renucci, 2001). fzd7b was originally referred to as either
Acknowledgements
We thank Carl-Philipp Heisenberg for kindly providing the fzd7a probe. We would also like to thank Chunyue Yin for technical advice and Rodney M. Dale and Elizabeth E. LeClair for critical comments of this manuscript. This work was supported by the National Institutes of Health - NIDCR Grants: R01DE016678 to J. Topczewski and F32DE019058 to B. Sisson.
References (35)
- et al.
Expression pattern of the frizzled 7 gene during zebrafish embryonic development
Mech. Dev.
(2001) - et al.
In vivo imaging of embryonic vascular development using transgenic zebrafish
Dev. Biol.
(2002) - et al.
Lateral line, nervous system, and maternal expression of Frizzled 7a during zebrafish embryogenesis
Mech. Dev.
(2002) - et al.
The zebrafish glypican knypek controls cell polarity during gastrulation movements of convergent extension
Dev. Cell
(2001) - et al.
Zebrafish frizzled7b is expressed in prechordal mesoderm, brain and paraxial mesoderm
Mech. Dev.
(2002) - et al.
Expression of frizzled genes in mouse costochondral chondrocytes
Matrix Biol.
(2001) - et al.
Wnt regulation of chondrocyte differentiation
J. Cell Sci.
(2002) - et al.
The emerging role of Wnt/PCP signaling in organ formation
Zebrafish
(2009) - et al.
Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles
Nat. Cell Biol.
(2008) - et al.
Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis
Development
(2000)
Expression of WNT signalling pathway genes during chicken craniofacial development
Dev. Dyn.
A genetic analysis of the determination of cuticular polarity during development in Drosophila melanogaster
J. Embryol. Exp. Morphol.
Frizzled6 controls hair patterning in mice
Proc. Natl. Acad. Sci. USA
Mutations affecting morphogenesis during gastrulation and tail formation in the zebrafish, Danio rerio
Development
Dual roles of Wnt signaling during chondrogenesis in the chicken limb
Development
Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation
Nature
Characterization of two frizzled8 homologues expressed in the embryonic shield and prechordal plate of zebrafish embryos
Mech. Dev.
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