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Fitoterapia
Volume 76, Issue 1, January 2005, Pages 62-66
 
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doi:10.1016/j.fitote.2004.09.021    How to Cite or Link Using DOI (Opens New Window)
Copyright © 2004 Elsevier B.V. All rights reserved.

Quantitative determination of α-tocopherol in Pistacia lentiscus, Pistacia lentiscus var. chia, and Pistacia terebinthus by TLC-densitometry and colorimetry

B. KıvçakCorresponding Author Contact Information, E-mail The Corresponding Author and S. Akay

Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey

Received 20 January 2003; 
accepted 24 September 2004. 
Available online 8 December 2004.

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Abstract

A quantitative determination of α-tocopherol in Pistacia lentiscus, Pistacia lentiscus var. chia, and Pistacia terebinthus, leaves was established by TLC-densitometry and colorimetry. The highest amount of α-tocopherol was found in P. lentiscus var. chia.

Keywords: Pistacia lentiscus; Pistacia lentiscus var. chia; Pistacia terebinthus; α-Tocopherol; TLC-densitometry; Colorimetry

Article Outline

1. Introduction
2. Experimental
2.1. Plant material
2.2. Extraction
2.3. Reagents and solvents
2.4. Sample solutions
2.5. TLC-densitometric assay
2.6. Colorimetric assay
2.6.1. 2,2′-Dipyridyl reagent
2.6.2. Ferric chloride reagent
2.6.3. Procedure
2.7. Statistical analysis
3. Results and discussion
Acknowledgements
References



Fitoterapia
Volume 76, Issue 1, January 2005, Pages 62-66
 
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