Inhibitory effects of metal ions on reductive dechlorination of polychlorinated biphenyls and perchloroethene in distinct organohalide-respiring bacteria

Highlights

• Organohalide respiration has relatively high tolerance to metal ions, among which Cd²⁺ is the most inhibitive heavy metal.

• No synergistic inhibition of metal ion mixtures on reductive dechlorination of PCE and PCB.

• Metal ions may have indirect inhibition on organohalide respiration through affecting non-dechlorinating populations.

Abstract

Bioremediation of sites co-contaminated with organohalides and metal pollutants may have unsatisfactory performance, since metal ions can potentially inhibit organohalide respiration. To understand the detailed impact of metals on organohalide respiration, we tested the effects of four metal ions (i.e., Cu²⁺, Cd²⁺, Cr³⁺ and Pb²⁺), as well as their mixtures, on reductive dechlorination of perchloroethene (PCE) and polychlorinated biphenyls (PCBs) in three different cultures, including a pure culture of Dehalococcoides mccartyi CG1, a Dehalococcoides-containing microcosm and a Dehalococcoides-Geobacter coculture. Results showed that the inhibitive impact on organohalide respiration depended on both the type and concentration of metal ions. Interestingly, the metal ions might indirectly inhibit organohalide respiration through affecting non-dechlorinating populations in the Dehalococcoides-containing microcosm. Nonetheless, compared to the CG1 pure culture, the Dehalococcoides-containing microcosm had higher tolerance to the individual metal ions. In addition, no synergistic inhibition was observed for reductive dechlorination of PCE and PCBs in cultures amended with metal ion mixtures. These results provide insights into the impact of metal ions on organohalide respiration, which may be helpful for future in situ bioremediation of organohalide-metal co-contaminated sites.