Introducing a novel bacterium, Vibrio neocaledonicus sp., with the highest corrosion inhibition efficiency

doi:10.1016/j.elecom.2014.12.007

Electrochemistry Communications

Volume 51, February 2015, Pages 64-68

https://doi.org/10.1016/j.elecom.2014.12.007 Get rights and content

Highlights

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A novel bacterium with the highest corrosion inhibitory effect is introduced.
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From EIS, corrosion resistance of carbon steel increased by more than sixty fold.
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An inhibitory layer covered the entire surface in the first hours of attachment.
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Extracellular polymeric substances of bacterium have a corrosion inhibitory effect.

Abstract

Degradation of metals due to corrosion causes serious economic problems throughout the world, and different corrosion protection techniques are being used to extend the service life of metallic structures. It has been suggested that some microorganisms can inhibit electrochemical corrosion of metals. Here we isolated a new marine inhibitory bacterium, Vibrio neocaledonicus sp., and EIS results showed that the corrosion resistance of carbon steel increased by more than sixty fold in the presence of this bacterium. This is the highest corrosion inhibitory effect reported for bacteria and is comparable with some industrial coatings such as electroless Ni. This bacterium affected corrosion by the formation of an inhibitory layer on the metal surface in the first hours of attachment, with the consumption of oxygen by electron transport proteins. Extracellular polymeric substances produced by this bacterium also have a corrosion inhibitory effect. Thus we propose a new, natural, non-toxic, and cost-effective system for controlling corrosion processes using this bacterium or extracellular polymeric substances produced by this bacterium.

Graphical abstract

Introduction

Marine steel structures, such as harbor and oil exploration facilities, become corroded over time and need to be renovated or modified, which is expensive. Currently, bio-based anti-corrosion agents are new substitutes for conventional products which are composed of biological products [1], [2], [3].

Due to the high interest for the application of natural, non-toxic and effective environmentally friendly materials as corrosion inhibitors instead of biocides or toxic evaporative organic compounds, the use of bacteria and their metabolic by-products including biofilm and extracellular polymeric substances (EPSs) is considered a lot. It has been suggested that bacteria and their metabolic by-products can eliminate corrosion-causing parameters, possibly due to the formation of biofilms that use up the oxygen which would otherwise be available to oxidize that metal [4], [5], [6], [7].

The first report on the corrosion inhibitory effect of bacteria was by Pedersen et al. [8]. Later, Jayaraman et al. [9] studied the mechanism underlying this process and reported that biofilm formation was crucial. A variety of bacteria have been isolated and their corrosion inhibitory effects were examined [10], [11], [12], [13]. The highest inhibitory effect of tenfold was found for Pseudomonas mendocina KR1 [6]. Different mechanisms have been proposed to explain corrosion inhibition by bacteria such as oxygen depletion on the metal surface because of biofilm formation [14]; formation of inorganic materials [15]; generation of antimicrobials by biofilms [16]; production of biofilm-secreted biosurfactant [17]; and corrosion inhibition by bacteriophages [18].

In this study, we present a new marine inhibitory bacterium, Vibrio neocaledonicus sp., with the highest corrosion inhibitory effect reported for bacteria. The mechanism and efficiency of corrosion inhibition were studied by different electrochemical, surface analysis and spectroscopic methods. To confirm the inhibitory effect of bacterium metabolic byproducts, EPS produced by this bacterium was extracted and examined.

Section snippets

Isolation and identification

Marine V. neocaledonicus sp. KJ841877 was isolated from marine sludge that was collected from the East Sea. Sampling, strain isolation, and culture procedures have been described previously [19]. This bacterium is deposited as MCCC 1K00266 in the Marine Culture Collection of China (MCCC), Xiamen, China. A strain culture is stored at − 80 °C in marine broth (MB) that contained 30% glycerol.

Materials

Squared-shaped carbon steel (ASTM A36) specimens with sides 15 mm thickness of 1 mm were used. A36 carbon steel

V. neocaledonicus sp. and its corrosion inhibition efficiency

A new generation of Vibrio strain, V. neocaledonicus sp. KJ841877 was isolated from the East Sea (Ningbo, China, GPS 29° 59′ 50.59″ N, 122° 2′ 34.99″ E) and after submitted to GenBank, deposited as MCCC 1K00266 in Marine Culture Collection of China (MCCC), Xiamen, China. This bacterium is a genus of Gram-negative bacteria that are typically found in saltwater. These bacteria have developed various strategies to survive under extreme conditions, such as metabolic pathway adaptations including

Conclusion

The study of the corrosion inhibitory effect of V. neocaledonicus sp. and EPS produced by this bacterium showed that the bacterium and its metabolic byproduct have a high inhibitory effect against corrosion of carbon steel. The inhibitory effect was caused by the formation of an inhibitory layer which covers the entire metal surfaces. The layer is composed of Fe–EPS complexes and strengthens by exposure time. We propose that V. neocaledonicus sp. and its EPS can be used as a new, natural,

Conflict of interest

There is no conflict of interest.

Acknowledgment

This work was supported by the National Natural Science Foundation of China (Grant No. 51301193) and China Postdoctoral Science Foundation (No. 2013M540503).

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Short communicationIntroducing a novel bacterium, Vibrio neocaledonicus sp., with the highest corrosion inhibition efficiency

Highlights

Abstract

Graphical abstract

Introduction

Section snippets

Isolation and identification

Materials

V. neocaledonicus sp. and its corrosion inhibition efficiency

Conclusion

Conflict of interest

Acknowledgment

J. Food Eng.

Colloid. Surf. A.

J. Microbiol. Methods

Electrochem. Commun.

Int. Biodeterior. Biodegrad.

Electrochim. Acta

Corros. Sci.

Corros. Sci.

Appl. Surf. Sci.

Thin Solid Films

Corros. Sci.

Mater. Chem. Phys.

Int. Biodeterior. Biodegrad.

Corros. Sci.

Electrochim. Acta

Corros. Sci.

Iron corrosion by novel anaerobic microorganisms

Nature

Short communication
Introducing a novel bacterium, Vibrio neocaledonicus sp., with the highest corrosion inhibition efficiency