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Accuracy of MIC determination for Streptococcus pneumoniae using the Sirscan2000automatic MIC determination system

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Abstract

The Sirscan2000automatic MIC determination (SIR-MD) system is a new system for MIC determination based on the automatic detection of growth of bacteria spotted onto agar medium using a camera scan. To evaluate its accuracy, 3608 Streptococcus pneumoniae clinical isolates yielding 18,165 MICs were tested in parallel with the SIR-MD and a standard interpretive antibiogram procedure. The overall percent agreement between the 2 methods within 1 log2 dilution was 86.9%. After exclusion of the 11.8% noninterpretable results, errors in the deduction of susceptibilities were very major in 0.03%, major in 0.2%, and minor in 1.3%.

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Acknowledgment

We thank Michel Roch for his valuable assistance concerning optimization of the system and the extraction of the data from the Sirscan.

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